Document Detail

Antimetastatic activity of Sulforaphane.
MedLine Citation:
PMID:  16600309     Owner:  NLM     Status:  MEDLINE    
The effect of Sulforaphane on the inhibition of lung metastasis induced by B16F-10 melanoma cells was studied in C57BL/6 mice by three different modalities of administration-simultaneous, prophylactic and after tumour developed. Of this simultaneous mode of Sulforaphane administration was found to be most effective. There was 95.5% inhibition of lung tumour nodule formation and 94.06% increase in the life span of metastatic tumour bearing animals. Highly elevated levels of lung hydroxyproline, lung uronic acid, lung hexosamine, serum sialic acid and serum gamma-glutamyl transpeptidase (GGT) in the metastatic control animals was found to be significantly lowered in the Sulforaphane treated animals. Histopathological analysis of lung tissues also correlated with these results. In the in vitro system Sulforaphane showed a significant inhibition in the invasion of B16F-10 melanoma cells across the collagen matrix. (3)H-thymidine proliferation assay showed that Sulforaphane could inhibit the proliferation of B16F-10 melanoma cells in vitro. Gelatin zymographic analysis showed that Sulforaphane could inhibit the activation of matrix metalloproteinases. These findings suggest that Sulforaphane reduced the invasion of B16F-10 melanoma cells by the inhibition of activation of matrix metalloproteinases, thereby inhibiting lung metastasis.
P Thejass; Girija Kuttan
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2006-04-04
Journal Detail:
Title:  Life sciences     Volume:  78     ISSN:  0024-3205     ISO Abbreviation:  Life Sci.     Publication Date:  2006 May 
Date Detail:
Created Date:  2006-05-10     Completed Date:  2006-06-14     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0375521     Medline TA:  Life Sci     Country:  England    
Other Details:
Languages:  eng     Pagination:  3043-50     Citation Subset:  IM    
Department of Immunology, Amala Cancer Research Centre, Amala Nagar, Thrissur, Kerala State 680555, India.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Brassica / chemistry
Cell Line
Cell Proliferation / drug effects
Collagen / metabolism
Electrophoresis, Polyacrylamide Gel
Hexosamines / metabolism
Hydroxyproline / metabolism
Lung / metabolism,  pathology
Lung Neoplasms / metabolism,  prevention & control,  secondary
Melanoma, Experimental / pathology
Mice, Inbred C57BL
N-Acetylneuraminic Acid / metabolism
Neoplasm Metastasis / prevention & control*
Survival Analysis
Tetrazolium Salts
Thiocyanates / pharmacology*
Thymidine / metabolism
Tumor Markers, Biological / metabolism
Uronic Acids / metabolism
gamma-Glutamyltransferase / metabolism
Reg. No./Substance:
0/Antineoplastic Agents; 0/Hexosamines; 0/Tetrazolium Salts; 0/Thiazoles; 0/Thiocyanates; 0/Tumor Markers, Biological; 0/Uronic Acids; 131-48-6/N-Acetylneuraminic Acid; 298-93-1/thiazolyl blue; 4478-93-7/sulforafan; 50-89-5/Thymidine; 51-35-4/Hydroxyproline; 9007-34-5/Collagen; EC

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