| Antilipopolysaccharide factor from horseshoe crab, Tachypleus tridentatus, inhibits lipopolysaccharide activation of cultured human endothelial cells. | |
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MedLine Citation:
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PMID: 2707859 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Antilipopolysaccharide (anti-LPS) factor is a basic protein that is purified from the hemocyte lysate of the Japanese and American horseshoe crabs (Tachypleus tridentatus and Limulus polyphemus). Anti-LPS factor has previously been reported to inhibit LPS-mediated activation of limulus factor C, lyse endotoxin-sensitized erythrocytes, and inhibit the growth of some gram-negative bacteria. In this study, we examine the ability of anti-LPS factor purified from T. tridentatus to inhibit the activation of cultured human endothelial cells by LPS. Anti-LPS factor inhibited the stimulation of endothelial adhesiveness for neutrophils by LPS in a dose-dependent manner. Maximum inhibition was achieved when anti-LPS factor was mixed with LPS prior to addition to the endothelial cell monolayers. Anti-LPS factor inhibited endothelial cell activation by LPS derived from Salmonella minnesota Re and Rc mutants as well as from the wild type (smooth), suggesting that it recognizes the lipid A moiety of LPS. |
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Authors:
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C E Desch; P O'Hara; J M Harlan |
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Publication Detail:
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Type: In Vitro; Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
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Title: Infection and immunity Volume: 57 ISSN: 0019-9567 ISO Abbreviation: Infect. Immun. Publication Date: 1989 May |
Date Detail:
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Created Date: 1989-06-01 Completed Date: 1989-06-01 Revised Date: 2009-11-18 |
Medline Journal Info:
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Nlm Unique ID: 0246127 Medline TA: Infect Immun Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1612-4 Citation Subset: IM |
Affiliation:
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Department of Medicine, University of Washington, Harborview Medical Center, Seattle 98104. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Cell Adhesion / drug effects Cells, Cultured Endothelium, Vascular / physiology* Enterobacteriaceae / physiology Hemolymph / analysis Horseshoe Crabs / analysis* Humans Lipopolysaccharides / antagonists & inhibitors* Neutrophils / physiology Proteins / pharmacology |
| Grant Support | |
ID/Acronym/Agency:
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HL03174/HL/NHLBI NIH HHS; HL30542/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Lipopolysaccharides; 0/Proteins |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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