Document Detail

Antilipoperoxidative and membrane stabilizing effect of diosgenin, in experimentally induced myocardial infarction.
MedLine Citation:
PMID:  19234676     Owner:  NLM     Status:  MEDLINE    
Altered membrane integrity has been suggested as a major factor in the development of cellular injury during myocardial necrosis. The present study was designed to investigate the effect of diosgenin on lysosomal hydrolases, membrane-bound enzymes, and electrolytes during isoproterenol (ISO)-induced myocardial necrosis in rats. Animals were pretreated with DIOS (80 mg/kg) for a period of 35 days. Myocardial infarction was experimentally induced with ISO (85 mg/kg) twice at 24 h interval. Experimental myocardial infarction was evidenced with marked elevation of creatine kinase-MB (CK-MB) in serum with concomitant increase in lipid peroxidation (plasma thiobarbituric acid reactive substances (TBARS) and hydroperoxides (HP)). Activity of lysosomal hydrolases (beta-glucuronidase, beta-N-acetyl glucosaminidase, beta-D-galactosidase, cathepsin D, and acid phosphatase) was found to be increased in serum and heart tissue of ISO-alone treated animals. DIOS (80 mg/kg) pretreated groups showed significant decrease in CK-MB, lipid peroxidation, and lysosomal hydrolases activity. The membrane-bound enzymes such as Ca2+-ATPase and Mg2+-ATPase activity was increased and Na+/K+-ATPase activity was decreased in the heart tissues of ISO-alone treated animals. These enzyme alterations lead to the change in the electrolytes content such as sodium, potassium, and calcium in the heart tissue. However, DIOS (80 mg/kg) pretreatment reversed the membrane-bound enzymes activity and thereby maintained the normal electrolyte concentration. These results suggest the protective action of diosgenin in ISO-induced myocardial infarction. The salubrious effect observed in this study might be due to the antioxidant and membrane stabilizing potential of diosgenin.
K S Jayachandran; Hannah R Vasanthi; G V Rajamanickam
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Publication Detail:
Type:  Journal Article     Date:  2009-02-21
Journal Detail:
Title:  Molecular and cellular biochemistry     Volume:  327     ISSN:  1573-4919     ISO Abbreviation:  Mol. Cell. Biochem.     Publication Date:  2009 Jul 
Date Detail:
Created Date:  2009-05-26     Completed Date:  2009-09-21     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0364456     Medline TA:  Mol Cell Biochem     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  203-10     Citation Subset:  IM    
Center for Advanced Research in Indian System of Medicine, SASTRA University, Thirumalaisamudram, Thanjavur 613402, Tamilnadu, India.
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MeSH Terms
Antioxidants / metabolism,  pharmacology*
Cell Membrane / drug effects,  enzymology*
Diosgenin / pharmacology*
Hydrolases / metabolism
Isoproterenol / pharmacology
Lipid Peroxidation
Lysosomes / enzymology,  metabolism
Models, Animal
Myocardial Infarction / chemically induced,  enzymology,  metabolism*
Rats, Wistar
Reg. No./Substance:
0/Antioxidants; 512-04-9/Diosgenin; 7683-59-2/Isoproterenol; EC 3.-/Hydrolases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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