Document Detail


Antigenicity of venous allografts.
MedLine Citation:
PMID:  106782     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
With isolated exceptions, the clinical use of venous allografts has been disappointing. Considerable evidence indicates that allograft antigenicity plays a major role in the failure of venous allografts when used as arterial replacements. Recent reports suggest that DMSO-cryopreservation of venous allografts may reduce allograft antigenicity while preserving allograft viability. The present study examines the effect of modifications of vein allografts on subsequent allograft antigenicity. Skin grafts were transplanted from ACI to Lewis inbred strains of male rats. Primary skin graft rejection occurred in 9.0 +/- 1.0 days. Subcutaneous implantation of fresh inferior vena cava from ACI rate into Lewis rats resulted in subsequent skin graft rejection in 5.0 +/- 1.0 days, confirming the antigenicity of venous tissue. Cryopreservation of ACI inferior vena cava for seven days prior to implantation, with or without 15% DMSO, resulted in subsequent skin graft rejection in 5.0 +/- 1.0 days. Treatment of ACI inferior vena cava with 0.30% gluteraldehyde for 20 minutes prior to implantation in Lewis rats resulted in skin graft rejection in 9.0 +/- 1.0 days, the same time as a first set rejection. This study indicates that unmodified veins are normally antigenic and that this antigenicity is not eliminated by cryopreservation with or without DMSO. Gluteraldehyde treatment appears to reduce allograft antigenicity, but results in a nonviable graft. At the present time, there is no known way to reduce the antigenicity of viable venous allografts.
Authors:
S C Axthelm; J M Porter; S Strickland; G M Baur
Publication Detail:
Type:  Journal Article; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Annals of surgery     Volume:  189     ISSN:  0003-4932     ISO Abbreviation:  Ann. Surg.     Publication Date:  1979 Mar 
Date Detail:
Created Date:  1979-05-23     Completed Date:  1979-05-23     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  0372354     Medline TA:  Ann Surg     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  290-3     Citation Subset:  AIM; IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens*
Cold Temperature
Dimethyl Sulfoxide / pharmacology
Freezing
Glutaral / pharmacology
Graft Rejection*
Histocompatibility
Humans
Rats
Rats, Inbred ACI
Rats, Inbred Lew
Skin / immunology
Skin Transplantation
Tissue Preservation / methods
Transplantation, Homologous
Veins / immunology,  transplantation*
Chemical
Reg. No./Substance:
0/Antigens; 111-30-8/Glutaral; 67-68-5/Dimethyl Sulfoxide
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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