Document Detail


Antigenicity of native and modified Kunitz soybean trypsin inhibitors.
MedLine Citation:
PMID:  2432759     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Food provides a continuous antigenic stimulus to the immune system and the antigenicity of processed food proteins should be considered in toxicological evaluations. The antigenicity of the Kunitz trypsin inhibitor was studied using antibodies prepared by inoculating rabbits with native, heat-denatured, and N-acetylcysteine-treated Kunitz soybean trypsin inhibitors. Immunochemical studies using a competitive solid-phase enzyme immunoassay and two groups of sera revealed two patterns of antigenicity. Antibodies elicited with the denatured inhibitor were specific for the denatured conformation of the protein. In contrast, native inhibitor elicited antibodies that selectively recognized determinants in both native and heat-treated protein, but that did not bind trypsin inhibitors treated with N-acetylcysteine. These results imply that: the disulfide bonds must be intact to maintain the native antigenic conformation and the cysteine treatment may suppress allergic manifestations of soybean trypsin inhibitors and possibly other food proteins. These studies were extended by analyzing a panel of monoclonal antibodies prepared against native Kunitz trypsin inhibitor. The inhibitor has at least two distinct antigenic sites (epitopes), one of which is retained under denaturing conditions. The measurement of native Kunitz trypsin inhibitor in food samples by immunoassay appears practical. The relevance of these findings to food processing, food safety, and health is also discussed.
Authors:
D L Brandon; S Haque; M Friedman
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Publication Detail:
Type:  Comparative Study; Journal Article    
Journal Detail:
Title:  Advances in experimental medicine and biology     Volume:  199     ISSN:  0065-2598     ISO Abbreviation:  Adv. Exp. Med. Biol.     Publication Date:  1986  
Date Detail:
Created Date:  1987-02-06     Completed Date:  1987-02-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0121103     Medline TA:  Adv Exp Med Biol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  449-67     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibodies
Antigen-Antibody Complex / analysis
Diet
Epitopes / analysis
Food Hypersensitivity*
Humans
Protein Denaturation
Soybean Proteins
Soybeans
Trypsin Inhibitor, Kunitz Soybean / immunology*,  toxicity
Trypsin Inhibitors / immunology*
Vegetable Proteins / immunology,  toxicity
Chemical
Reg. No./Substance:
0/Antibodies; 0/Antigen-Antibody Complex; 0/Epitopes; 0/Soybean Proteins; 0/Trypsin Inhibitors; 0/Vegetable Proteins; 9088-41-9/Trypsin Inhibitor, Kunitz Soybean

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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