| Antigenicity of native and modified Kunitz soybean trypsin inhibitors. | |
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MedLine Citation:
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PMID: 2432759 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Food provides a continuous antigenic stimulus to the immune system and the antigenicity of processed food proteins should be considered in toxicological evaluations. The antigenicity of the Kunitz trypsin inhibitor was studied using antibodies prepared by inoculating rabbits with native, heat-denatured, and N-acetylcysteine-treated Kunitz soybean trypsin inhibitors. Immunochemical studies using a competitive solid-phase enzyme immunoassay and two groups of sera revealed two patterns of antigenicity. Antibodies elicited with the denatured inhibitor were specific for the denatured conformation of the protein. In contrast, native inhibitor elicited antibodies that selectively recognized determinants in both native and heat-treated protein, but that did not bind trypsin inhibitors treated with N-acetylcysteine. These results imply that: the disulfide bonds must be intact to maintain the native antigenic conformation and the cysteine treatment may suppress allergic manifestations of soybean trypsin inhibitors and possibly other food proteins. These studies were extended by analyzing a panel of monoclonal antibodies prepared against native Kunitz trypsin inhibitor. The inhibitor has at least two distinct antigenic sites (epitopes), one of which is retained under denaturing conditions. The measurement of native Kunitz trypsin inhibitor in food samples by immunoassay appears practical. The relevance of these findings to food processing, food safety, and health is also discussed. |
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Authors:
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D L Brandon; S Haque; M Friedman |
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Publication Detail:
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Type: Comparative Study; Journal Article |
Journal Detail:
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Title: Advances in experimental medicine and biology Volume: 199 ISSN: 0065-2598 ISO Abbreviation: Adv. Exp. Med. Biol. Publication Date: 1986 |
Date Detail:
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Created Date: 1987-02-06 Completed Date: 1987-02-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0121103 Medline TA: Adv Exp Med Biol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 449-67 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies Antigen-Antibody Complex / analysis Diet Epitopes / analysis Food Hypersensitivity* Humans Protein Denaturation Soybean Proteins Soybeans Trypsin Inhibitor, Kunitz Soybean / immunology*, toxicity Trypsin Inhibitors / immunology* Vegetable Proteins / immunology, toxicity |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Antigen-Antibody Complex; 0/Epitopes; 0/Soybean Proteins; 0/Trypsin Inhibitors; 0/Vegetable Proteins; 9088-41-9/Trypsin Inhibitor, Kunitz Soybean |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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