| Antigenicity of adult Schistosoma mansoni alkaline phosphatase. | |
| | |
MedLine Citation:
|
PMID: 2108424 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Antibodies to the alkaline phosphatase (AP) of Schistosoma mansoni in infected human and mice sera were evaluated by a direct solid-phase AP immunoadsorption assay (APIA) and by Western blot and immunostaining. APIA consisted of (a) solid-phase capture of immunoglobulins from infected human or mice, (b) immunoadsorption of the enzyme antigen by the antibodies, and (c) detection of the enzymatic activity. By this procedure the appearance of the anti-AP response in mice was detected around 50 days post-infection; this response was not specific for an AP of a given schistosome strain and it was not induced by an autoimmunity phenomenon. Fourteen out of 15 sera from infected people tested by APIA showed a clear antibody response against this enzyme. Immunoblots in non-reducing conditions supported APIA results indicating that the parasite AP was specifically recognized by the antibodies present in infected human and mice sera. These results suggest the possible usefulness of the schistosome AP as a marker for S. mansoni infection. |
| | |
Authors:
|
F H Pujol; I M Cesari |
Related Documents
:
|
16605124 - Application of adult worm and lung-stage antigens to immunize against schistosoma manso... 9921344 - Immunization with piii, a fraction of schistosoma mansoni soluble adult worm antigenic ... 11812494 - Litomosoides sigmodontis cystatin acts as an immunomodulator during experimental filari... 14500704 - Localization and identification of schistosoma mansoni/klh-crossreactive components in ... 20036064 - Toxic shock syndrome toxin-1 (tsst-1) antibody levels in japanese children. 3170994 - Inflammatory changes in conjunctival scrapings after allergen provocation in humans. |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
|
Title: Parasite immunology Volume: 12 ISSN: 0141-9838 ISO Abbreviation: Parasite Immunol. Publication Date: 1990 Mar |
Date Detail:
|
Created Date: 1990-05-10 Completed Date: 1990-05-10 Revised Date: 2006-11-15 |
Medline Journal Info:
|
Nlm Unique ID: 7910948 Medline TA: Parasite Immunol Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 189-98 Citation Subset: IM |
Affiliation:
|
Laboratorio de Imnunoparasitologia, Instituto Venezolano de Investigaciones Científicas (IVIC), Caracas, Venezuela. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Alkaline Phosphatase
/
immunology* Animals Antibodies, Helminth / biosynthesis* Antigens, Helminth / immunology Blotting, Western Humans Immunoassay Mice Schistosoma mansoni / enzymology*, immunology Schistosomiasis mansoni / immunology* |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Helminth; 0/Antigens, Helminth; EC 3.1.3.1/Alkaline Phosphatase |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Intraoperative facial nerve monitoring. Technical aspects.
Next Document: Pulsatile secretion of LH and FSH in prepubertal and early pubertal boys revealed by ultrasensitive ...