| Antigenic protein modifications in Ehrlichia. | |
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MedLine Citation:
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PMID: 19493209 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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To develop effective vaccination strategies against Ehrlichia, we have previously reported developing an animal model of cross-protection in which C57BL/6 mice primed with E. muris were resistant to lethal infection with Ixodes ovatus ehrlichia (IOE). Polyclonal antibody produced in mice after priming with E. muris and later injected with IOE-detected antigenic proteins in E. muris and IOE cell lysates. Cross-reaction of antigenic proteins was observed when we probed both the E. muris and IOE cell lysates with IOE and E. muris-specific polyclonal antibody. Analysis of the total proteins of E. muris and IOE by two dimensional electrophoresis showed that both E. muris and IOE have the same antigenic proteins. Finally, studies on post-translational protein modifications using a novel technique, Eastern blotting, showed that E. muris proteins are more lipoylated and glycosylated than those of IOE. |
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Authors:
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S Thomas; N Thirumalapura; E C Crossley; N Ismail; D H Walker |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Parasite immunology Volume: 31 ISSN: 1365-3024 ISO Abbreviation: Parasite Immunol. Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-06-04 Completed Date: 2009-07-16 Revised Date: 2011-08-01 |
Medline Journal Info:
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Nlm Unique ID: 7910948 Medline TA: Parasite Immunol Country: England |
Other Details:
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Languages: eng Pagination: 296-303 Citation Subset: IM |
Affiliation:
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Department of Pathology, Center for Biodefense and Emerging Infectious Diseases, University of Texas Medical Branch, Galveston, Texas, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antigens, Bacterial / analysis, immunology* Bacterial Proteins / analysis, immunology* Ehrlichia / chemistry, immunology* Electrophoresis, Gel, Two-Dimensional Ixodes / microbiology* Mice Mice, Inbred C57BL Protein Processing, Post-Translational Proteome / analysis |
| Grant Support | |
ID/Acronym/Agency:
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AI031431/AI/NIAID NIH HHS; R01 AI031431-16/AI/NIAID NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antigens, Bacterial; 0/Bacterial Proteins; 0/Proteome |
| Comments/Corrections | |
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