Document Detail


Antigenic properties of estriol 3-glucuronide-[C-6]-bovine serum albumin conjugates having oxime bridges.
MedLine Citation:
PMID:  6655549     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
The preparation and antigenic properties of estriol 3-glucuronide-bovine serum albumin (BSA) conjugate in which the hapten is linked to the carrier protein through an (O-3-carboxypropylcarbamoylmethyl)oxime bridge at the C-6 position on the steroid nucleus, has been described. 16,17-Di-O-acetyl-6-oxoestriol 6-(O-carboxymethyl)oxime 3-glucuronide acetate-methyl ester was condensed with gamma-amino-n-butyric acid by the mixed anhydride method. Subsequent coupling with BSA followed by removal of the protecting groups with alkali gave the desired hapten-BSA conjugate. The antisera elicited in the rabbit with the conjugate were highly specific to estriol 3-glucuronide, discriminating from other 3-substituted compounds. The specificity of antisera improved by elongation of the 6-(O-carboxymethyl)oxime bridge has been discussed.
Authors:
T Nambara; T Ohkubo; K Shimada
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Journal of pharmacobio-dynamics     Volume:  6     ISSN:  0386-846X     ISO Abbreviation:  J. Pharmacobio-dyn.     Publication Date:  1983 Sep 
Date Detail:
Created Date:  1984-02-24     Completed Date:  1984-02-24     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7901854     Medline TA:  J Pharmacobiodyn     Country:  JAPAN    
Other Details:
Languages:  eng     Pagination:  692-7     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antibody Specificity
Antigens*
Cattle
Chemical Phenomena
Chemistry
Cross Reactions
Estriol / analogs & derivatives*,  immunology
Oximes
Serum Albumin, Bovine / immunology
Chemical
Reg. No./Substance:
0/Antigens; 0/Oximes; 0/Serum Albumin, Bovine; 2479-91-6/estriol 3-glucuronide; 50-27-1/Estriol

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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