| Antigenic properties of estriol 3-glucuronide-[C-6]-bovine serum albumin conjugates having oxime bridges. | |
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MedLine Citation:
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PMID: 6655549 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The preparation and antigenic properties of estriol 3-glucuronide-bovine serum albumin (BSA) conjugate in which the hapten is linked to the carrier protein through an (O-3-carboxypropylcarbamoylmethyl)oxime bridge at the C-6 position on the steroid nucleus, has been described. 16,17-Di-O-acetyl-6-oxoestriol 6-(O-carboxymethyl)oxime 3-glucuronide acetate-methyl ester was condensed with gamma-amino-n-butyric acid by the mixed anhydride method. Subsequent coupling with BSA followed by removal of the protecting groups with alkali gave the desired hapten-BSA conjugate. The antisera elicited in the rabbit with the conjugate were highly specific to estriol 3-glucuronide, discriminating from other 3-substituted compounds. The specificity of antisera improved by elongation of the 6-(O-carboxymethyl)oxime bridge has been discussed. |
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Authors:
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T Nambara; T Ohkubo; K Shimada |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Journal of pharmacobio-dynamics Volume: 6 ISSN: 0386-846X ISO Abbreviation: J. Pharmacobio-dyn. Publication Date: 1983 Sep |
Date Detail:
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Created Date: 1984-02-24 Completed Date: 1984-02-24 Revised Date: 2008-11-21 |
Medline Journal Info:
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Nlm Unique ID: 7901854 Medline TA: J Pharmacobiodyn Country: JAPAN |
Other Details:
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Languages: eng Pagination: 692-7 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibody Specificity Antigens* Cattle Chemical Phenomena Chemistry Cross Reactions Estriol / analogs & derivatives*, immunology Oximes Serum Albumin, Bovine / immunology |
| Chemical | |
Reg. No./Substance:
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0/Antigens; 0/Oximes; 0/Serum Albumin, Bovine; 2479-91-6/estriol 3-glucuronide; 50-27-1/Estriol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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