| Antigenic and physicochemical characterization of the 2nd International Standard for hepatitis B virus surface antigen (HBsAg). | |
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MedLine Citation:
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PMID: 20117963 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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BACKGROUND: Standard preparations for HBsAg are required for quality control of test kits and clinical studies on HBsAg quantitation. WHO provides purified heat inactivated HBsAg diluted in negative defribinated plasma as 2nd International Standard (IS) for quality control of tests. OBJECTIVE: Study of possible alterations of antigenicity, protein composition, size and density of the heat inactivated source material (SM) for the 2nd IS. STUDY DESIGN: Native HBsAg and SM were examined by quantitative immune electrophoresis (QIE), SDS-PAGE, ultracentrifugation and gel chromatography. HBV DNA was sequenced and the HBsAg geno/subtype derived. RESULTS: The SM contained 97,600 International Units HBsAg/ml in QIE which agreed very well with the previous evaluations by WHO using 10 different assays. In SDS-PAGE, SM showed on a strong background the small HBs proteins but no preS proteins. SM had a more heterogeneous density than native HBsAg and contained particle aggregates. The HBsAg geno/subtype of SM was A2/adw2. CONCLUSIONS: The IS has very good HBs antigenicity, but it lacks the preS domains, has modified HBs proteins and is partially aggregated. While it has been proven very useful for quality control of tests, certain inconsistencies due to the altered structure of its HBsAg cannot be excluded. |
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Authors:
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Christian G Sch?ttler; Ulrike C Wend; Fabian M Faupel; P Nico Lelie; Wolfram H Gerlich |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't Date: 2010-02-01 |
Journal Detail:
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Title: Journal of clinical virology : the official publication of the Pan American Society for Clinical Virology Volume: 47 ISSN: 1873-5967 ISO Abbreviation: J. Clin. Virol. Publication Date: 2010 Mar |
Date Detail:
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Created Date: 2010-02-22 Completed Date: 2010-04-30 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9815671 Medline TA: J Clin Virol Country: Netherlands |
Other Details:
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Languages: eng Pagination: 238-42 Citation Subset: IM |
Affiliation:
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Institute for Medical Virology, Justus Liebig University, Frankfurter Str. 107, 35392 Giessen, Germany. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chromatography, Gel Electrophoresis, Polyacrylamide Gel Hepatitis B / diagnosis* Hepatitis B Surface Antigens / chemistry*, immunology* Humans Immunoassay / standards* Reference Standards Ultracentrifugation Virology / methods* |
| Chemical | |
Reg. No./Substance:
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0/Hepatitis B Surface Antigens |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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