Document Detail

Antigenic phenotype and functional characterization of human tonsil B cells.
MedLine Citation:
PMID:  2451545     Owner:  NLM     Status:  MEDLINE    
Human tonsil cells were labeled with anti-leu-16, a monoclonal antibody (MoAb) that recognizes the CD20 antigen and that is specific for B cells. Two populations of B cells were identified by flow cytometry on the basis of antigen density. One labeled brightly with anti-Leu-16, and the other labeled at a level comparable to blood B cells. These two populations were characterized with a panel of MoAbs in two- and three-color flow cytometric studies and appeared to correspond to germinal-center and mantle-zone B cells. The pattern of staining of anti-Leu-16 on sections of frozen tonsil supported this characterization. Anti-Leu-16 labeled germinal center cells more intensely than mantle zone cells and stained a few scattered B cells in the interfollicular zone. The ability of each Leu-16+ population to secrete IgG and IgM in response to mitogens was measured in a particle immunofluorescence assay. Dim Leu-16+ B cells (small, resting B cells and a subpopulation of preactivated cells) secreted IgG and IgM in response to pokeweed mitogen (PWM) but only IgG in response to B cell growth factor (BCGF). Bright Leu-16+ B cells (small to large activated cells and possibly memory cells) did not respond to PWM but secreted IgG in response to BCGF. The functional responses of dim Leu-16+ and bright Leu-16+ B cells were consistent with their identification as mantle-zone and germinal-center B cells. Phenotypic identification and functional studies of mantle-zone and germinal-center B cells may help clarify the differentiation pathway within the germinal center.
N Gadol; M A Peacock; K A Ault
Related Documents :
20853345 - Dynamic proliferation assessment in flow cytometry.
4509645 - Bromodeoxyuridine dependence--a new mutation in mammalian cells.
17186265 - Replication of segment-specific and intercalated cells in the mouse renal collecting sy...
12907855 - Neurogenesis in the hippocampus of an adult marsupial.
11346885 - Inhibitory effect of silibinin on ligand binding to erbb1 and associated mitogenic sign...
17248985 - Rare-cell fusion events between diploid and haploid strains of the sexually agglutinati...
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Blood     Volume:  71     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1988 Apr 
Date Detail:
Created Date:  1988-05-17     Completed Date:  1988-05-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1048-55     Citation Subset:  AIM; IM    
Becton Dickinson Immunocytometry Systems, Mountain View, CA 94043.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Antibodies, Monoclonal
Antigens, Differentiation, B-Lymphocyte / analysis,  immunology
Antigens, Surface / analysis*,  immunology
B-Lymphocytes / classification*,  immunology,  physiology
Cell Separation
Flow Cytometry
HLA-D Antigens / analysis
Lymphocyte Function-Associated Antigen-1
Palatine Tonsil / analysis,  anatomy & histology,  cytology*
Receptors, Antigen, B-Cell / analysis
Staining and Labeling
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Differentiation, B-Lymphocyte; 0/Antigens, Surface; 0/HLA-D Antigens; 0/Lymphocyte Function-Associated Antigen-1; 0/Receptors, Antigen, B-Cell; 0/Suspensions

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

Previous Document:  Functional effects of monoclonal antibodies (mAbs) against human polymorphonuclear (PMN) adherence a...
Next Document:  The population of paroxysmal nocturnal hemoglobinuria neutrophils deficient in decay-accelerating fa...