Document Detail


Antigenic phenotype and functional characterization of human tonsil B cells.
MedLine Citation:
PMID:  2451545     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Human tonsil cells were labeled with anti-leu-16, a monoclonal antibody (MoAb) that recognizes the CD20 antigen and that is specific for B cells. Two populations of B cells were identified by flow cytometry on the basis of antigen density. One labeled brightly with anti-Leu-16, and the other labeled at a level comparable to blood B cells. These two populations were characterized with a panel of MoAbs in two- and three-color flow cytometric studies and appeared to correspond to germinal-center and mantle-zone B cells. The pattern of staining of anti-Leu-16 on sections of frozen tonsil supported this characterization. Anti-Leu-16 labeled germinal center cells more intensely than mantle zone cells and stained a few scattered B cells in the interfollicular zone. The ability of each Leu-16+ population to secrete IgG and IgM in response to mitogens was measured in a particle immunofluorescence assay. Dim Leu-16+ B cells (small, resting B cells and a subpopulation of preactivated cells) secreted IgG and IgM in response to pokeweed mitogen (PWM) but only IgG in response to B cell growth factor (BCGF). Bright Leu-16+ B cells (small to large activated cells and possibly memory cells) did not respond to PWM but secreted IgG in response to BCGF. The functional responses of dim Leu-16+ and bright Leu-16+ B cells were consistent with their identification as mantle-zone and germinal-center B cells. Phenotypic identification and functional studies of mantle-zone and germinal-center B cells may help clarify the differentiation pathway within the germinal center.
Authors:
N Gadol; M A Peacock; K A Ault
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Blood     Volume:  71     ISSN:  0006-4971     ISO Abbreviation:  Blood     Publication Date:  1988 Apr 
Date Detail:
Created Date:  1988-05-17     Completed Date:  1988-05-17     Revised Date:  2008-11-21    
Medline Journal Info:
Nlm Unique ID:  7603509     Medline TA:  Blood     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  1048-55     Citation Subset:  AIM; IM    
Affiliation:
Becton Dickinson Immunocytometry Systems, Mountain View, CA 94043.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Monoclonal
Antigens, Differentiation, B-Lymphocyte / analysis,  immunology
Antigens, Surface / analysis*,  immunology
B-Lymphocytes / classification*,  immunology,  physiology
Cell Separation
Flow Cytometry
HLA-D Antigens / analysis
Humans
Immunohistochemistry
Lymphocyte Function-Associated Antigen-1
Palatine Tonsil / analysis,  anatomy & histology,  cytology*
Phenotype
Receptors, Antigen, B-Cell / analysis
Staining and Labeling
Suspensions
Chemical
Reg. No./Substance:
0/Antibodies, Monoclonal; 0/Antigens, Differentiation, B-Lymphocyte; 0/Antigens, Surface; 0/HLA-D Antigens; 0/Lymphocyte Function-Associated Antigen-1; 0/Receptors, Antigen, B-Cell; 0/Suspensions

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