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Antigenic and cross-protection studies of biotype 1 and biotype 2 isolates of Yersinia ruckeri in rainbow trout, Oncorhynchus mykiss(Walbaum).
MedLine Citation:
PMID:  21447021     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Aims:  The study investigated antigen characteristics of biotype (bt) 1 and bt 2 isolates of Yersinia ruckeri. Methods and Results:  The cell surface characteristics of Y. ruckeri were compared for their antigenic characteristics using polyclonal antibodies that revealed that both biotypes had a homogenous whole cell protein antigenic profile. Notable differences in the antigenic properties were observed in the LPS profile of both biotypes. Two iron regulated outer membrane proteins (IROMP) of ∼ 90 kDa and ∼ 100 kDa were shown to be major specific antigens. The results demonstrate for the first time differences in antigens between bt 1 and 2 isolates of serotype O1 isolates of Y. ruckeri. The protection induced in rainbow trout by a commercial monovalent and bivalent inactivated vaccine was tested with the outcome that the ability of isolates to cause mortality in vaccinated fish varied with geographic location. In this context, vaccination studies suggested that the O antigen was the dominant immunogenic molecule involved in protection against the disease. Conclusions:  The O-antigen of Y. ruckeri was the dominant immunogenic molecule involved in protection of rainbow trout against enteric redmouth disease. Significance and Impact of the Study:  There are distinct phenotypic and antigenic differences in Y. ruckeri bt 1 and bt 2 with O-antigen recognised as the dominant immunogenic molecule. The data have significance in explaining the lack of success of the earlier monovalent vaccine, and demonstrate the effectiveness of the newer bivalent vaccine.
Authors:
J W Tinsley; A R Lyndon; B Austin
Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2011-3-29
Journal Detail:
Title:  Journal of applied microbiology     Volume:  -     ISSN:  1365-2672     ISO Abbreviation:  -     Publication Date:  2011 Mar 
Date Detail:
Created Date:  2011-3-30     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Journal compilation © 2011 The Society for Applied Microbiology.
Affiliation:
School of Life Sciences, Heriot-Watt University, Edinburgh, U.K. Institute of Aquaculture, University of Stirling, Stirling, UK.
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