| Antigen-specific T cell suppressor factor (TsF): isolation of a cDNA clone encoding for a functional polypeptide chain of phosphorylcholine-specific TsF. | |
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MedLine Citation:
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PMID: 2412834 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A cDNA library of phosphorylcholine (PC)-specific suppressor T hybridoma, 29-C-6, was constructed. By differential colony hybridization, 68 colonies were selected which hybridized with mRNA probes of two PC-specific suppressor T hybridomas, 29-C-6 and 09-M-24-8, but not with those of BW5147. A hybridization translation assay revealed that only one combination of translation products of cDNA clones, p6-1 and p6-5, showed strong PC-T cell suppressor factor (TsF) activity. Sequence analysis showed that p6-5 contained a specific cDNA sequence of about 800 base pairs (bp) while p6-1 had a 190 bp poly(A) sequence insert. When total poly(A) + RNA of 29-C-6 was hybridized with p6-1 filter alone the recovered mRNA was capable of producing PC-TsF. However, when the total poly-(A) + RNA was hybridized with a p6-1 filter combined with a p6-5 filter, the mRNA eluted from the p6-1 filter was not able to produce PC-TsF, suggesting that the depletion of p6-5 specific mRNA from p6-1-binding poly(A) + RNA led to a complete abolishment of the capability to produce PC-TsF. Furthermore, p6-5 hybridizing mRNA could successfully restore the p6-1-binding poly(A) + RNA depleted of p6-5-specific mRNA, and translation products of both RNA mixtures showed strong PC-TsF activity. These results suggest that PC-TsF is not a single polypeptide chain, but is composed of at least two distinct polypeptide chains, and also that p6-5 contains a cDNA sequence encoding for one of the polypeptide chains composing the PC-TsF molecule. |
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Authors:
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K Sugimura; N Yamasaki; M Matsuura; T Watanabe |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: European journal of immunology Volume: 15 ISSN: 0014-2980 ISO Abbreviation: Eur. J. Immunol. Publication Date: 1985 Sep |
Date Detail:
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Created Date: 1985-11-06 Completed Date: 1985-11-06 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1273201 Medline TA: Eur J Immunol Country: GERMANY, WEST |
Other Details:
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Languages: eng Pagination: 873-80 Citation Subset: IM; Q |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Base Sequence Cell Separation Choline / analogs & derivatives* DNA / metabolism Epitopes Hybridomas / immunology*, metabolism Mice Mice, Inbred BALB C Nucleic Acid Hybridization* Oocytes / metabolism Peptide Fragments / immunology Phosphorylcholine / immunology* Protein Biosynthesis RNA, Messenger / metabolism Suppressor Factors, Immunologic / genetics*, metabolism T-Lymphocytes / immunology*, metabolism Xenopus |
| Chemical | |
Reg. No./Substance:
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0/Epitopes; 0/Peptide Fragments; 0/RNA, Messenger; 0/Suppressor Factors, Immunologic; 107-73-3/Phosphorylcholine; 62-49-7/Choline; 9007-49-2/DNA |
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