Document Detail

Antigen-specific T cell suppressor factor (TsF): isolation of a cDNA clone encoding for a functional polypeptide chain of phosphorylcholine-specific TsF.
MedLine Citation:
PMID:  2412834     Owner:  NLM     Status:  MEDLINE    
A cDNA library of phosphorylcholine (PC)-specific suppressor T hybridoma, 29-C-6, was constructed. By differential colony hybridization, 68 colonies were selected which hybridized with mRNA probes of two PC-specific suppressor T hybridomas, 29-C-6 and 09-M-24-8, but not with those of BW5147. A hybridization translation assay revealed that only one combination of translation products of cDNA clones, p6-1 and p6-5, showed strong PC-T cell suppressor factor (TsF) activity. Sequence analysis showed that p6-5 contained a specific cDNA sequence of about 800 base pairs (bp) while p6-1 had a 190 bp poly(A) sequence insert. When total poly(A) + RNA of 29-C-6 was hybridized with p6-1 filter alone the recovered mRNA was capable of producing PC-TsF. However, when the total poly-(A) + RNA was hybridized with a p6-1 filter combined with a p6-5 filter, the mRNA eluted from the p6-1 filter was not able to produce PC-TsF, suggesting that the depletion of p6-5 specific mRNA from p6-1-binding poly(A) + RNA led to a complete abolishment of the capability to produce PC-TsF. Furthermore, p6-5 hybridizing mRNA could successfully restore the p6-1-binding poly(A) + RNA depleted of p6-5-specific mRNA, and translation products of both RNA mixtures showed strong PC-TsF activity. These results suggest that PC-TsF is not a single polypeptide chain, but is composed of at least two distinct polypeptide chains, and also that p6-5 contains a cDNA sequence encoding for one of the polypeptide chains composing the PC-TsF molecule.
K Sugimura; N Yamasaki; M Matsuura; T Watanabe
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of immunology     Volume:  15     ISSN:  0014-2980     ISO Abbreviation:  Eur. J. Immunol.     Publication Date:  1985 Sep 
Date Detail:
Created Date:  1985-11-06     Completed Date:  1985-11-06     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  1273201     Medline TA:  Eur J Immunol     Country:  GERMANY, WEST    
Other Details:
Languages:  eng     Pagination:  873-80     Citation Subset:  IM; Q    
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MeSH Terms
Base Sequence
Cell Separation
Choline / analogs & derivatives*
DNA / metabolism
Hybridomas / immunology*,  metabolism
Mice, Inbred BALB C
Nucleic Acid Hybridization*
Oocytes / metabolism
Peptide Fragments / immunology
Phosphorylcholine / immunology*
Protein Biosynthesis
RNA, Messenger / metabolism
Suppressor Factors, Immunologic / genetics*,  metabolism
T-Lymphocytes / immunology*,  metabolism
Reg. No./Substance:
0/Epitopes; 0/Peptide Fragments; 0/RNA, Messenger; 0/Suppressor Factors, Immunologic; 107-73-3/Phosphorylcholine; 62-49-7/Choline; 9007-49-2/DNA

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