Document Detail


Antigen receptor engagement delivers a stop signal to migrating T lymphocytes.
MedLine Citation:
PMID:  9108078     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
We investigated the role of the T cell antigen receptor (TcR) in control of T cell migration in an in vitro system. We used T cells from transgenic mice bearing a TcR for the lysozyme peptide 48-62 bound to I-A(k) (3A9). T cells from the 3A9 TcR transgenic mice crawled on purified intercellular adhesion molecule-1 substrates, but strikingly, stopped upon interaction with the physiological ligand, i.e., the mouse I-A(k) with covalently attached hen egg white lysozyme peptide residues 48-62 complex. TcR-triggered stopping was reversible by treatment with adhesion-strengthening phorbol esters. The microtubule organizing center of stopped cells was positioned adjacent to the site of stable cell anchorage. Direct conversion of lymphocyte function associated-1 to the high-affinity conformation with antibodies also stopped T cells in a similar manner to antigen. Thus, physiological TcR engagement triggers a stop signal through lymphocyte function associated-1. We propose that the stop signal is an early and essential event in T cell activation that also will play an important role in control of T cell migration.
Authors:
M L Dustin; S K Bromley; Z Kan; D A Peterson; E R Unanue
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S.    
Journal Detail:
Title:  Proceedings of the National Academy of Sciences of the United States of America     Volume:  94     ISSN:  0027-8424     ISO Abbreviation:  Proc. Natl. Acad. Sci. U.S.A.     Publication Date:  1997 Apr 
Date Detail:
Created Date:  1997-05-22     Completed Date:  1997-05-22     Revised Date:  2013-04-16    
Medline Journal Info:
Nlm Unique ID:  7505876     Medline TA:  Proc Natl Acad Sci U S A     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  3909-13     Citation Subset:  IM    
Affiliation:
Center for Immunology and Department of Pathology, Washington University School of Medicine, St. Louis, MO 63110, USA. dustin@pathbox.wustl.edu
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MeSH Terms
Descriptor/Qualifier:
Animals
Cell Movement / immunology*
Mice
Mice, Transgenic
Receptors, Antigen, T-Cell / immunology*
Signal Transduction / immunology*
T-Lymphocytes / cytology,  immunology*
Chemical
Reg. No./Substance:
0/Receptors, Antigen, T-Cell
Comments/Corrections

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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