Document Detail


Antigen-loaded pH-sensitive hydrogel microparticles are taken up by dendritic cells with no requirement for targeting antibodies.
MedLine Citation:
PMID:  23060228     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Particle-based delivery of encapsulated antigens has great potential for improving vaccine constructs. In this study, we show that antigen-loaded, pH-sensitive hydrogel microparticles are taken up and presented by bone marrow-derived dendritic cells (BMDCs) in vitro and are taken up by dendritic cells (DCs) and monocytes in vivo. This uptake is irrespective of targeting antibodies. BMDCs in vitro and DCs in vivo also display upregulation of activation markers CD80 and CD86 when treated with microparticles, again with no difference in conjugated antibodies, even the agonistic CD40 antibody. We further show that these particles induce enhanced expansion of cytokine-producing CD8 T cells in response to challenge with ovalbumin-expressing vesicular stomatitis virus, in both an accelerated vaccination strategy using pre-loaded BMDCs and a traditional mouse immunization setting.
Authors:
Laura E Ruff; Enas A Mahmoud; Jagadis Sankaranarayanan; José M Morachis; Carol D Katayama; Maripat Corr; Stephen M Hedrick; Adah Almutairi
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Publication Detail:
Type:  Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Integrative biology : quantitative biosciences from nano to macro     Volume:  5     ISSN:  1757-9708     ISO Abbreviation:  Integr Biol (Camb)     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2012-12-17     Completed Date:  2013-06-24     Revised Date:  2014-04-08    
Medline Journal Info:
Nlm Unique ID:  101478378     Medline TA:  Integr Biol (Camb)     Country:  England    
Other Details:
Languages:  eng     Pagination:  195-203     Citation Subset:  IM    
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MeSH Terms
Descriptor/Qualifier:
Animals
Antigens / administration & dosage*,  immunology*
Cells, Cultured
Delayed-Action Preparations / chemistry*
Dendritic Cells / immunology*
Hydrogels / chemistry*
Hydrogen-Ion Concentration
Mice
Mice, Inbred C57BL
Microspheres
Grant Support
ID/Acronym/Agency:
1 DP2 OD006499-01/OD/NIH HHS; DP2 OD006499/OD/NIH HHS; GM06852/GM/NIGMS NIH HHS; K12 GM068524/GM/NIGMS NIH HHS; T32AI060536/AI/NIAID NIH HHS
Chemical
Reg. No./Substance:
0/Antigens; 0/Delayed-Action Preparations; 0/Hydrogels
Comments/Corrections

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