| Antigen-induced and polyclonal B-cell responses in human peripheral blood lymphocyte cultures. | |
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MedLine Citation:
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PMID: 3494539 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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This work was designed to delineate the anti-hapten antibody (Ab) response induced by trinitrophenol-polyacrylamide (TNP-PAA) beads from the nonspecific B-cell response which concomitantly occurs in human peripheral blood mononuclear cell (PBMC) cultures. Indeed human PBMC produce consistent amounts of immunoglobulins when cultured at high cell density in the presence of fetal bovine serum, regardless of the presence of antigen. In contrast, the stimulation of such cultures by TNP-PAA leads to an Ab response characterized by the following: cells secreting anti-hapten Ab at a high rate (detected by a plaque-forming cel (PFC) assay); a 10-30 times enhancement in the number of hapten-specific binding cells (detected by a rosette-forming cell (RFC) assay); the production of anti-TNP IgM Ab (detected by an ELISA assay). The anti-TNP response is specifically triggered by the particulate antigen, as shown by the following: The TNP-PAA antigen induces a clear-cut increase in the amount of anti-TNP Ab whereas it only marginally increases that of total IgM. The anti-TNP Ab response is specifically abolished when anti-TNP RFC are depleted from the PBMC preparation before the initiation of the cultures. The anti-TNP Ab response is specifically abolished when PBMC are triggered by TNP-PAA in the concomitant presence of a soluble TNP-protein conjugate. These results demonstrate the ability of polymeric antigens to specifically activate human peripheral blood B cells. |
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Authors:
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P Galanaud; M C Crevon; J F Delfraissy; M T Rannou; Y Richard; D Emilie; A Vazquez |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Cellular immunology Volume: 106 ISSN: 0008-8749 ISO Abbreviation: Cell. Immunol. Publication Date: 1987 May |
Date Detail:
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Created Date: 1987-06-23 Completed Date: 1987-06-23 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 1246405 Medline TA: Cell Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 234-41 Citation Subset: IM |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Acrylic Resins Antibody Formation* Antibody Specificity Antigens, T-Independent / immunology* B-Lymphocytes / immunology* Cells, Cultured Clone Cells / immunology Haptens Humans Immunoglobulin G / biosynthesis Immunoglobulin M / biosynthesis Receptors, Antigen, B-Cell / analysis Rosette Formation Time Factors Trinitrobenzenes / immunology |
| Chemical | |
Reg. No./Substance:
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0/Acrylic Resins; 0/Antigens, T-Independent; 0/Haptens; 0/Immunoglobulin G; 0/Immunoglobulin M; 0/Receptors, Antigen, B-Cell; 0/Trinitrobenzenes; 9003-05-8/polyacrylamide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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