| Antigen expression in biofilm cells of Aeromonas hydrophila employed in oral vaccination of fish. | |
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MedLine Citation:
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PMID: 15123309 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Total protein, S-layer protein and lipopolysaccharides (LPS) of biofilm cells of Aeromonas hydrophila were analysed by SDS-PAGE and compared with that of planktonic cells. In the whole cell lysate of biofilm cells, about 15 proteins were repressed while three new proteins were expressed compared to that in planktonic cells. Interestingly, in biofilm cells the S-layer proteins were lost and LPS showed an additional high molecular weight band compared to that in planktonic cells. We propose that the change in LPS profile must have contributed to the loss of S-layer. Also, the high molecular weight band of LPS might play a role in the better performance of biofilm oral vaccine by eliciting a protective immune response. |
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Authors:
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A Asha; D K Nayak; K M Shankar; C V Mohan |
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Publication Detail:
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Type: Comparative Study; Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: Fish & shellfish immunology Volume: 16 ISSN: 1050-4648 ISO Abbreviation: Fish Shellfish Immunol. Publication Date: 2004 Mar |
Date Detail:
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Created Date: 2004-05-04 Completed Date: 2004-06-07 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9505220 Medline TA: Fish Shellfish Immunol Country: England |
Other Details:
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Languages: eng Pagination: 429-36 Citation Subset: IM |
Affiliation:
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Fish Pathology and Biotechnology Laboratory, Department of Aquaculture, College of Fisheries, University of Agricultural Sciences, Mangalore 575 002, India. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Aeromonas hydrophila
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chemistry*,
immunology* Aquaculture / methods Bacterial Proteins / analysis* Biofilms* Electrophoresis, Polyacrylamide Gel Lipopolysaccharides / analysis* Proteins / analysis* Vaccination / methods* |
| Chemical | |
Reg. No./Substance:
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0/Bacterial Proteins; 0/Lipopolysaccharides; 0/Proteins; 0/S-layer-encoding structural protein, Aeromonas hydrophila |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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