| Antifungal activity of amphotericin B, caspofungin and posaconazole on Candida albicans biofilms in intermediate and mature development phases. | |
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MedLine Citation:
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PMID: 19298353 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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The aim of this study was to examine the antifungal activity of amphotericin B, caspofungin and posaconazole on Candida albicans biofilms in the intermediate and mature development phases. Candida albicans biofilms, previously grown for either 24, 48 or 72 h in 96-well microtitre plates, were treated for 48 h with amphotericin B, caspofungin or posaconazole in increasing concentrations according to the respective minimal inhibitory concentration (MIC) determined for planktonic cells (1-128 x MIC). The biofilms were quantified using the mean optical density (OD) determined by XTT assay. Antifungal activities were expressed as percentage of reduction in OD of drug-treated biofilms compared to untreated biofilms. To test the fungicidal activity of antifungal agents, the unfixed biofilms were scraped off and seeded to Sabouraud agar. Caspofungin and amphotericin B showed higher activity against C. albicans biofilm grown for 24 h and 72 h (>or=50% reduction of OD) than biofilms grown for 48 h, whereas posaconazole showed similar, but reduced activity against all phases of C. albicans biofilm (<or=50% reduction of OD). Caspofungin at 1-4 x MIC achieved the greatest decrease in the biofilm OD grown for 24, 48 and 72 h, whereas amphotericin B showed dose-dependent activity. However, all tested antifungals failed to reach fungicidal activity in all biofilm development phases. |
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Authors:
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Selma Tobudic; Andrea Lassnigg; Christina Kratzer; Wolfgang Graninger; Elisabeth Presterl |
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Publication Detail:
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Type: Journal Article Date: 2009-02-26 |
Journal Detail:
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Title: Mycoses Volume: 53 ISSN: 1439-0507 ISO Abbreviation: Mycoses Publication Date: 2010 May |
Date Detail:
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Created Date: 2010-05-17 Completed Date: 2010-08-09 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8805008 Medline TA: Mycoses Country: Germany |
Other Details:
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Languages: eng Pagination: 208-14 Citation Subset: IM |
Affiliation:
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Department of Internal Medicine I, Division of Infectious Diseases and Tropical Medicine, Medical University of Vienna, Austria. selma.tobudic@meduniwien.ac.at |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Amphotericin B
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pharmacology* Antifungal Agents / pharmacology* Biofilms / drug effects* Candida albicans / drug effects* Colony Count, Microbial Echinocandins / pharmacology* Humans Microbial Sensitivity Tests Microbial Viability / drug effects Triazoles / pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Antifungal Agents; 0/Echinocandins; 0/Triazoles; 0/caspofungin; 0/posaconazole; 1397-89-3/Amphotericin B |
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