| Antifreeze protein genes of the winter flounder. | |
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MedLine Citation:
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PMID: 6086629 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A genomic library of the winter flounder in lambda phage Charon 4 was screened with antifreeze protein cDNA. Nine genomic clones were isolated from three haploid genome equivalents of phage and were grouped into three classes on the basis of restriction mapping and heteroduplex analysis. The three chromosomal regions are non-overlapping and each contain two antifreeze protein genes spaced from 3 to 7 kilobase pairs apart. Genomic Southern blots indicate that these six non-allelic genes represent only 10-20% of the complement of this large multigene family. One gene has been identified by sequencing as a variant of the gene encoding the most abundant antifreeze protein. This gene is 1.0 kilobase pair long and contains an intervening sequence of 0.6 kilobase pairs between the coding region for the bulk of the signal sequence and that coding for the proprotein. |
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Authors:
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P L Davies; C Hough; G K Scott; N Ng; B N White; C L Hew |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: The Journal of biological chemistry Volume: 259 ISSN: 0021-9258 ISO Abbreviation: J. Biol. Chem. Publication Date: 1984 Jul |
Date Detail:
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Created Date: 1984-08-27 Completed Date: 1984-08-27 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 2985121R Medline TA: J Biol Chem Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 9241-7 Citation Subset: IM |
| Data Bank Information | |
Bank Name/Acc. No.:
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GENBANK/J00929; L00138; N00014 |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Amino Acid Sequence Animals Antifreeze Proteins Base Sequence Cloning, Molecular DNA / isolation & purification DNA Restriction Enzymes Fishes / metabolism* Freezing Genes* Glycoproteins / genetics* Microscopy, Electron Nucleic Acid Hybridization Plasmids |
| Chemical | |
Reg. No./Substance:
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0/Antifreeze Proteins; 0/Glycoproteins; 9007-49-2/DNA; EC 3.1.21.-/DNA Restriction Enzymes |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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