Document Detail

Antiendomysial and antihuman recombinant tissue transglutaminase antibodies in the diagnosis of coeliac disease: a biopsy-proven European multicentre study.
MedLine Citation:
PMID:  15647647     Owner:  NLM     Status:  MEDLINE    
OBJECTIVE: To investigate the value of serum antitissue transglutaminase IgA antibodies (IgA-TTG) and IgA antiendomysial antibodies (IgA-EMA) in the diagnosis of coeliac disease in cohorts from different geographical areas in Europe. The setting allowed a further comparison between the antibody results and the conventional small-intestinal histology. METHODS: A total of 144 cases with coeliac disease [median age 19.5 years (range 0.9-81.4)], and 127 disease controls [median age 29.2 years (range 0.5-79.0)], were recruited, on the basis of biopsy, from 13 centres in nine countries. All biopsy specimens were re-evaluated and classified blindly a second time by two investigators. IgA-TTG were determined by ELISA with human recombinant antigen and IgA-EMA by an immunofluorescence test with human umbilical cord as antigen. RESULTS: The quality of the biopsy specimens was not acceptable in 29 (10.7%) of 271 cases and a reliable judgement could not be made, mainly due to poor orientation of the samples. The primary clinical diagnosis and the second classification of the biopsy specimens were divergent in nine cases, and one patient was initially enrolled in the wrong group. Thus, 126 coeliac patients and 106 controls, verified by biopsy, remained for final analysis. The sensitivity of IgA-TTG was 94% and IgA-EMA 89%, the specificity was 99% and 98%, respectively. CONCLUSIONS: Serum IgA-TTG measurement is effective and at least as good as IgA-EMA in the identification of coeliac disease. Due to a high percentage of poor histological specimens, the diagnosis of coeliac disease should not depend only on biopsy, but in addition the clinical picture and serology should be considered.
Pekka Collin; Katri Kaukinen; Harald Vogelsang; Ilma Korponay-Szabó; Rudolf Sommer; Elisabeth Schreier; Umberto Volta; Alessandro Granito; Lorenza Veronesi; Françoise Mascart; Annick Ocmant; Anneli Ivarsson; Carina Lagerqvist; Annemarie Bürgin-Wolff; Faruk Hadziselimovic; Raoul I Furlano; Marc A Sidler; Chris J J Mulder; Marije S Goerres; M Luisa Mearin; Maarten K Ninaber; Eivind Gudmand-Høyer; Elisabetta Fabiani; Carlo Catassi; Helena Tidlund; Lisbeth Alainentalo; Markku Mäki
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Publication Detail:
Type:  Comparative Study; Evaluation Studies; Journal Article; Multicenter Study; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  European journal of gastroenterology & hepatology     Volume:  17     ISSN:  0954-691X     ISO Abbreviation:  Eur J Gastroenterol Hepatol     Publication Date:  2005 Jan 
Date Detail:
Created Date:  2005-01-13     Completed Date:  2005-04-12     Revised Date:  2009-10-16    
Medline Journal Info:
Nlm Unique ID:  9000874     Medline TA:  Eur J Gastroenterol Hepatol     Country:  England    
Other Details:
Languages:  eng     Pagination:  85-91     Citation Subset:  IM    
Tampere University Hospital and University of Tampere, Finland.
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MeSH Terms
Aged, 80 and over
Autoantibodies / blood*
Biological Markers / blood
Celiac Disease / diagnosis*,  pathology
Child, Preschool
Duodenum / pathology
Immunoglobulin A / blood
Middle Aged
Muscle Fibers, Skeletal / immunology*
Recombinant Proteins / immunology
Sensitivity and Specificity
Transglutaminases / immunology*
Reg. No./Substance:
0/Autoantibodies; 0/Biological Markers; 0/Immunoglobulin A; 0/Recombinant Proteins; EC
Comment In:
Eur J Gastroenterol Hepatol. 2005 Jan;17(1):41-3   [PMID:  15647639 ]

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