Document Detail


Antidiabetic effects of bitter gourd extracts in insulin-resistant db/db mice.
MedLine Citation:
PMID:  20615270     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Bitter gourd (BG, Momordica charantia) exerts proven blood glucose- and body weight-lowering effects. To develop an effective and safe application, it is necessary to identify the bioactive compounds and biochemical mechanisms responsible for these effects in type 2 diabetes. A total of forty-five 4-week-old male db/db mice were assigned to five groups of nine each. The mice were given sterile tap water as a control, a whole fruit powder, the lipid fraction, the saponin fraction or the hydrophilic residue of BG at a daily oral dosage of 150 mg/kg body weight for 5 weeks, respectively. Weight gain was significantly decreased in all the BG-treated groups (P ≤ 0.05). Glycated Hb levels were the highest in the control mice compared with all the four BG-treated mice (P = 0.02). The lipid fraction had the strongest effect, and it tended (P = 0.075) to reduce glycated Hb levels from 9.3 % (control mice) to 8.0 % (lipid fraction-treated mice). The lipid and saponin fractions reduced lipid peroxidation of adipose tissue significantly (P ≤ 0.01). Additionally, the saponin fraction and the lipid fraction reduced protein tyrosine phosphatase 1B (PTP 1B) activity in skeletal muscle cytosol by 25 % (P = 0.05) and 23 % (P = 0.07), respectively. PTP 1B is the physiological antagonist of the insulin signalling pathway. Inhibition of PTP 1B increases insulin sensitivity. This is the first study to demonstrate that BG is involved in PTP 1B regulation, and thus explains one possible biochemical mechanism underlying the antidiabetic effects of BG in insulin resistance and type 2 diabetes.
Authors:
Sandra D Klomann; Andreas S Mueller; Josef Pallauf; Michael B Krawinkel
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2010-07-09
Journal Detail:
Title:  The British journal of nutrition     Volume:  104     ISSN:  1475-2662     ISO Abbreviation:  Br. J. Nutr.     Publication Date:  2010 Dec 
Date Detail:
Created Date:  2010-12-01     Completed Date:  2011-01-04     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0372547     Medline TA:  Br J Nutr     Country:  England    
Other Details:
Languages:  eng     Pagination:  1613-20     Citation Subset:  IM    
Affiliation:
Institute of Nutritional Sciences, Justus Liebig University Giessen, Giessen, Germany. sandra.d.klomann@ernaehrung.uni-giessen.de
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MeSH Terms
Descriptor/Qualifier:
Adipose Tissue / drug effects
Animals
Cytosol / drug effects,  metabolism
Diabetes Mellitus, Type 2 / drug therapy*,  metabolism
Fruit
Hemoglobin A, Glycosylated / metabolism
Hypoglycemic Agents / pharmacology,  therapeutic use*
Insulin Resistance* / genetics
Lipid Peroxidation / drug effects
Lipids / pharmacology,  therapeutic use
Male
Mice
Mice, Knockout
Momordica / chemistry*
Muscle, Skeletal / drug effects,  metabolism
Phytotherapy
Plant Preparations / pharmacology,  therapeutic use*
Protein Tyrosine Phosphatase, Non-Receptor Type 1 / metabolism*
Saponins / pharmacology,  therapeutic use
Weight Gain / drug effects*
Chemical
Reg. No./Substance:
0/Hemoglobin A, Glycosylated; 0/Hypoglycemic Agents; 0/Lipids; 0/Plant Preparations; 0/Saponins; EC 3.1.3.48/Protein Tyrosine Phosphatase, Non-Receptor Type 1

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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