| Anticoagulant properties of the anti-inflammatory cytokine IL-10 in a factor Xa-activated human monocyte model. | |
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MedLine Citation:
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PMID: 23137705 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: Monocytes and factor Xa (FXa) are procoagulant agents implicated in the physio-pathological processes of atherosclerosis and thrombosis. Objective: we evaluated the anticoagulant effect of the anti-inflammatory cytokine IL-10 on an FXa-activated human monocyte (Hu-monocyte) procoagulant phenotype. Methods: Hu-monocytes were purified by elutriation and activated by FXa. The effect of IL-10 was assessed by means of a 2 h pre-incubation step with recombined human IL-10 (0.5 and 1 ng/mL). Real-time RT-PCR and Western blotting were used to evaluate the effect of IL-10 on tissue factor (TF) mRNA and protein synthesis. A thrombin generation (TG) assay was used as a functional test to assess the effect of IL-10 on TF-dependent TG. Results: we showed that IL-10 inhibited both TF mRNA and TF protein expression in a dose-dependant manner. We showed, as a functional consequence, that IL-10 inhibited Hu-monocyte-triggered TG and that this inhibition was concentration-dependant, and significant for all TG phases. The rate index of the propagation phase (rate index) was the most sensitive parameter while the endpoint of TG decay (S-tail) and the endogenous thrombin potential (ETP) were the least sensitive (inhibition of 80, 40 and 30% respectively). The IL-10 pattern of TG inhibition was similar to TF-Ab-induced inhibition: IC(50 )were not reached by ETP and S-tail, and the lowest IC(50 )values were reached by the rate index (0.61 ± 0.12 ng/mL and 1.87 ± 0.35 μg/mL respectively). Conclusion: the anticoagulant effect of the anti-inflammatory cytokine IL-10 in an FXa-activated Hu-monocyte model is an additional illustration of the cross-talk between inflammation and coagulation, opening new approaches in the field of arteriosclerosis and thrombosis. |
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Authors:
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Sonia Ben-Hadj-Khalifa; Philippe Nguyen; Touhami Mahjoub; Nathalie Hézard |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-11-9 |
Journal Detail:
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Title: European cytokine network Volume: - ISSN: 1952-4005 ISO Abbreviation: Eur. Cytokine Netw. Publication Date: 2012 Nov |
Date Detail:
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Created Date: 2012-11-9 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9100879 Medline TA: Eur Cytokine Netw Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Unité de recherche des maladies hématologiques et auto-immunes, faculté de pharmacie, 5000 Monastir, Tunisie, Laboratoire d'hématologie, CHU Robert Debré, 51092 Reims Cedex, France. |
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