| Anticoagulant-dependent in vitro hemagglutination in a cat. | |
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MedLine Citation:
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PMID: 19490570 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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A 17-year-old domestic shorthaired cat was presented to the Cornell University Hospital for Animals for recheck of hyperthyroidism previously treated with radioiodine. Marked agglutination was noted in a blood sample collected into EDTA for a CBC; no other clinical or hematologic evidence of hemolysis was observed and none developed despite persistent agglutination in additional EDTA samples collected over a 2-month period. Blood drawn into heparin and sodium citrate tubes did not have grossly or microscopically detectable agglutination, unless EDTA was added. Plasma from the cat induced agglutination of washed RBCs from a control cat in the presence of EDTA but not in the presence of heparin. Flow cytometric analysis of samples created by mixing plasma from the patient with washed RBCs from a control cat showed immunoglobulin coating of the control RBCs, predominantly by IgM. These findings suggested an anticoagulant-dependent antibody-mediated mechanism for the agglutination. EDTA-dependent hemagglutination has not been reported previously in cats, although rare cases have been described in humans. The phenomenon needs to be recognized as an in vitro occurrence in order to prevent erroneous diagnosis of immune-mediated hemolytic anemia. |
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Authors:
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Deanna M W Schaefer; Heather Priest; Tracy Stokol; Richard E Goldstein; Nicole Belcher |
Publication Detail:
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Type: Case Reports; Journal Article Date: 2009-03-12 |
Journal Detail:
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Title: Veterinary clinical pathology / American Society for Veterinary Clinical Pathology Volume: 38 ISSN: 0275-6382 ISO Abbreviation: Vet Clin Pathol Publication Date: 2009 Jun |
Date Detail:
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Created Date: 2009-07-27 Completed Date: 2009-09-01 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9880575 Medline TA: Vet Clin Pathol Country: United States |
Other Details:
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Languages: eng Pagination: 194-200 Citation Subset: IM |
Affiliation:
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Departments of Population Medicine and Diagnostic Sciences, Cornell University College of Veterinary Medicine, Ithaca, NY 14853, USA. dms279@cornell.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Anticoagulants / chemistry* Cats / blood* Chelating Agents / chemistry* Edetic Acid / chemistry* Female Hemagglutination Tests / veterinary* Heparin / chemistry* |
| Chemical | |
Reg. No./Substance:
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0/Anticoagulants; 0/Chelating Agents; 60-00-4/Edetic Acid; 9005-49-6/Heparin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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