Document Detail


Anticoagulant activity of a sulfated chitosan.
MedLine Citation:
PMID:  12110200     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Chitin prepared from the shells of rice-field crabs (Somanniathelphusa dugasti) was converted into chitosan with a degree of acetylation of 0.21 and then sulfated with chlorosulfonic acid in N,N-dimethylformamide under semi-heterogeneous conditions to give 87% of water-soluble sulfated chitosan with degree of substitution (d.s) of 2.13. 1H NMR revealed the sulfate substitution at C-2, C-3 and C-6. Gel filtration on Sepharose CL-6B of the sulfated chitosan gave three fractions with average molecular weights of 7.1, 3.5, and 1.9 x 10(4). The three sulfated chitosan preparations showed strong anticoagulant activities, with the same mechanism of action observed for standard therapeutic heparin.
Authors:
Preeyanat Vongchan; Warayuth Sajomsang; Dumrat Subyen; Prachya Kongtawelert
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Carbohydrate research     Volume:  337     ISSN:  0008-6215     ISO Abbreviation:  Carbohydr. Res.     Publication Date:  2002 Jul 
Date Detail:
Created Date:  2002-07-11     Completed Date:  2003-06-30     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0043535     Medline TA:  Carbohydr Res     Country:  Netherlands    
Other Details:
Languages:  eng     Pagination:  1239-42     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, Faculty of Medicine, Chiang Mai University, Chiang Mai, 50200, Thailand. pvongchan@hotmail.com
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MeSH Terms
Descriptor/Qualifier:
Acetylation
Animals
Anticoagulants / chemical synthesis,  chemistry*,  isolation & purification,  pharmacology*
Blood Coagulation / drug effects
Chitin / analogs & derivatives*,  chemical synthesis,  chemistry*,  isolation & purification,  pharmacology*
Chitosan
Chromatography, Gel
Heparin / pharmacology
Humans
Magnetic Resonance Spectroscopy
Molecular Structure
Sulfates / chemistry*
Sulfonic Acids / chemistry
Thrombin / antagonists & inhibitors
Chemical
Reg. No./Substance:
0/Anticoagulants; 0/Sulfates; 0/Sulfonic Acids; 1398-61-4/Chitin; 7790-94-5/chlorosulfonic acid; 9005-49-6/Heparin; 9012-76-4/Chitosan; EC 3.4.21.5/Thrombin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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