Document Detail


Anticancer properties of Ganoderma lucidum methanol extracts in vitro and in vivo.
MedLine Citation:
PMID:  19838944     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Anticancer activities of various extracts of the medicinal mushroom, Ganoderma lucidum, have been widely demonstrated and are mainly associated with the presence of different bioactive polysaccharides and triterpenoids. We have evaluated and compared in vitro and in vivo the antitumor effects of two preparations from Ganoderma lucidum: a methanol extract containing total terpenoids (GLme) and a purified methanol extract containing mainly acidic terpenoids (GLpme). Both extracts inhibited tumor growth of B16 mouse melanoma cells inoculated subcutaneously into syngeneic C57BL/6 mice and reduced viability of B16 cells in vitro, whereby GLme exhibited stronger effect. Furthermore, anticancer activity of GLme was demonstrated for the first time against two other rodent tumor cell lines, L929-mouse fibrosarcoma and C6-rat astrocytoma. The mechanism of antitumor activity of GLme comprised inhibition of cell proliferation and induction of caspase-dependent apoptotic cell death mediated by upregulated p53 and inhibited Bcl-2 expression. Moreover, the antitumor effect of the GLme was associated with intensified production of reactive oxygen species, whereas their neutralization by the antioxidant, N-acetyl cysteine, resulted in partial recovery of cell viability. Thus, our results suggest that GLme might be a good candidate for treatment of diverse forms of cancers.
Authors:
Ljubica M Harhaji Trajkovi??; Sanja A Mijatovi??; Danijela D Maksimovi??-Ivani??; Ivana D Stojanovi??; Miljana B Momcilovi??; Srdjan J Tufegdzi??; Vuk M Maksimovi??; Zaklina S Marjanovi??; Stanislava D Stosi??-Grujici??
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Nutrition and cancer     Volume:  61     ISSN:  1532-7914     ISO Abbreviation:  Nutr Cancer     Publication Date:  2009  
Date Detail:
Created Date:  2009-10-19     Completed Date:  2010-01-15     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  7905040     Medline TA:  Nutr Cancer     Country:  United States    
Other Details:
Languages:  eng     Pagination:  696-707     Citation Subset:  IM    
Affiliation:
Institute for Biological Research Sinisa Stankovi??, Belgrade University, Belgrade, Serbia.
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MeSH Terms
Descriptor/Qualifier:
Animals
Antineoplastic Agents / chemistry,  metabolism,  pharmacology*
Apoptosis / drug effects
Astrocytes / pathology
Caspases / metabolism
Cell Cycle / drug effects
Cell Line, Tumor
Cell Survival / drug effects
Cells, Cultured
Drugs, Chinese Herbal / chemistry,  metabolism,  pharmacology*
Gene Expression Regulation, Neoplastic
Melanoma, Experimental / drug therapy*
Mice
Mice, Inbred C57BL
Necrosis / chemically induced
Neoplasm Transplantation
Oxidative Stress / drug effects
Rats
Reactive Oxygen Species / analysis,  antagonists & inhibitors
Reishi / chemistry*
Serbia
Terpenes / analysis
Tumor Burden / drug effects
Chemical
Reg. No./Substance:
0/Antineoplastic Agents; 0/Drugs, Chinese Herbal; 0/Reactive Oxygen Species; 0/Terpenes; EC 3.4.22.-/Caspases

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