Document Detail


Anticancer effect of aloe-emodin on cervical cancer cells involves G2/M arrest and induction of differentiation.
MedLine Citation:
PMID:  18031614     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
AIM: The aim of this study was to investigate the effects of aloe-emodin, a natural compound from the root and rhizome of Rheum palmatum, on the growth of human cervical cancer cells, HeLa. METHODS: HeLa cells were treated with various concentrations of aloe-emodin for 1-5 d, and cell growth was measured by 3-(4, 5-dimethylthiazol-2-yl)-2, 5-diphenyl tetrazolium bromide assay. The long-term growth effect was investigated by crystal violet assay. The distributions of the cell cycle and apoptosis were analyzed by flow cytometry. The alkaline phosphatase (ALP) activity was analyzed by a chemical analyzer. Finally, Western blotting was used to indicate the abundant changes of protein kinase C (PKC), c-myc, cyclins, cyclin-dependent kinases (CDK), and proliferating cell nuclear antigen (PCNA). RESULTS: Aloe-emodin inhibited the growth of HeLa cells in a dose-dependent manner at concentrations ranging between 2.5 and 40 micromol/L. The flow cytometric analysis showed that HeLa cells were arrested at the G2/M phase. This effect was associated with the decrease in cyclin A and CDK2, and the increase in cyclin B1 and CDK1. More importantly, the ALP activity was found to be increased by aloe-emodin treatment, and accompanied by the inhibition of PCNA expression. In addition, aloe-emodin suppressed the expression of PKCalpha and c-myc. CONCLUSION: These findings provide a possible mechanistic explanation for the growth inhibitory effect of aloe-emodin on HeLa, which includes cell cycle arrest and inducing differentiation.
Authors:
Jun-ming Guo; Bing-xiu Xiao; Qiong Liu; Shun Zhang; Dong-hai Liu; Zhao-hui Gong
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  Acta pharmacologica Sinica     Volume:  28     ISSN:  1671-4083     ISO Abbreviation:  Acta Pharmacol. Sin.     Publication Date:  2007 Dec 
Date Detail:
Created Date:  2007-11-22     Completed Date:  2009-04-27     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  100956087     Medline TA:  Acta Pharmacol Sin     Country:  China    
Other Details:
Languages:  eng     Pagination:  1991-5     Citation Subset:  IM    
Affiliation:
Ningbo University School of Medicine, Ningbo 315211, China. junmingguo@yahoo.com
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MeSH Terms
Descriptor/Qualifier:
Anthraquinones / pharmacology*
Cell Differentiation / drug effects*
Cell Division / drug effects*
Female
G2 Phase / drug effects*
Hela Cells
Humans
Uterine Cervical Neoplasms / pathology*
Chemical
Reg. No./Substance:
0/Anthraquinones; 0/aloe emodin

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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