| Anticancer activities of the ruthenium carboxylato, amido and pyridine complexes. | |
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MedLine Citation:
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PMID: 20428784 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Ruthenium complexes [1-3] with oxalato, amido and pyridine ligands have been synthesized and characterized. Biological tests showed that the ruthenium complexes 2 and 3 have modest cytotoxicity on both murine cell line NIH3T3 and human cancer colon cell lines HCT116 and HT29 while complex 1 has no obvious growth defect. We further tested why complexes 2 and 3 exhibit modest cytotoxicity. Gel electrophoresis analysis demonstrates that complex 3 has the ability to cleave double-stranded DNAs in a dose-dependent manner. In contrast, complex 2 does not have detectable DNA cleavage activity. Additionally, real-time PCR analysis suggests that the observed cell growth inhibition or death could be due to the altered gene expression in the processes of cell cycle and apoptosis/necrosis caused by the addition of complexes 2 and 3. Furthermore, cell death by DNA fragmentation assay indicates that the addition of these two complexes may cause cell necrosis rather than apoptosis. |
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Authors:
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Radhey S Srivastava; Frank R Fronczek; Richard S Perkins; Tomofusa Fukuyama; Wu Xu |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't |
Journal Detail:
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Title: International journal of oncology Volume: 36 ISSN: 1791-2423 ISO Abbreviation: Int. J. Oncol. Publication Date: 2010 Jun |
Date Detail:
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Created Date: 2010-04-29 Completed Date: 2010-08-20 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: Greece |
Other Details:
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Languages: eng Pagination: 1591-8 Citation Subset: IM |
Affiliation:
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Department of Chemistry, University of Louisiana at Lafayette, LA 70504, USA. rss1805@louisiana.edu |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antineoplastic Agents / chemical synthesis*, chemistry, pharmacology* Cell Proliferation / drug effects Cell Survival / drug effects DNA / drug effects* DNA Fragmentation / drug effects HCT116 Cells HT29 Cells Humans Mice NIH 3T3 Cells Pyridines / chemical synthesis, chemistry, pharmacology Reverse Transcriptase Polymerase Chain Reaction Ruthenium Compounds / chemical synthesis*, chemistry, pharmacology* |
| Chemical | |
Reg. No./Substance:
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0/Antineoplastic Agents; 0/Pyridines; 0/Ruthenium Compounds; 9007-49-2/DNA |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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