| Antibody to E- and L-selectin does not prevent lung injury or mortality in septic baboons. | |
| | |
MedLine Citation:
|
PMID: 9517615 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
Recruitment of polymorphonuclear leukocytes (PMN) through upregulation of cellular adhesion molecules is a proposed mechanism of injury in sepsis and acute respiratory distress syndrome (ARDS). We hypothesized that pretreatment of baboons with a monoclonal antibody to human E- and L-selectin (EL-246) during sepsis would decrease PMN influx into tissues and result in less organ injury during gram-negative sepsis. We studied 14 anesthetized, ventilated adult baboons; six animals received 1 mg/kg of EL-246 before infusion of an LD100 of live Escherichia coli and six received the E. coli infusion without antibody therapy. Two other animals received 1 mg/kg of EL-246 intravenously without an infusion of bacteria. Intermittent measurements were made of circulatory pressures, cardiac output, urine output, arterial blood gases, ventilation:perfusion ratio (VA/Q), and hematologic status. The experiments were ended at 48 h or at the time of death. Tissues were harvested for pathology and biochemical measurements. The E. coli infusions were associated with a hyperdynamic state, pulmonary hypertension, systemic hypotension, decreased urine output (UOP), and metabolic acidosis. The antibody partly blocked PMN migration, but there were few significant physiologic or biochemical differences between the EL-246-treated and untreated animals. In the antibody-treated animals, UOP was decreased, metabolic acidosis was worsened, and median survival time was decreased significantly. We conclude that treatment with an antibody to E- and L-selectin in gram-negative sepsis does not improve gas exchange or protect against lung injury, and is associated with decreased survival time in primates. |
| | |
Authors:
|
M S Carraway; K E Welty-Wolf; S P Kantrow; Y C Huang; S G Simonson; L G Que; T K Kishimoto; C A Piantadosi |
Related Documents
:
|
1391265 - Lung mechanics (frc and static pressure-volume diagram) after endotracheal surfactant i... 6893755 - Hypobaric hypoxia on pulmonary wash fluid of rats. 6389455 - Effects of surfactant supplement and end-expiratory pressure in lung-lavaged rabbits. 16545795 - Polymyxin b immobilized column is effective for hydrochloric acid-induced lung injury i... 2686855 - Eicosanoids and the hemodynamic course of live escherichia coli-induced sepsis in baboons. 1724535 - Effects of nitroglycerin and nitroprusside on vascular capacitance of anesthetized gang... |
Publication Detail:
|
Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: American journal of respiratory and critical care medicine Volume: 157 ISSN: 1073-449X ISO Abbreviation: Am. J. Respir. Crit. Care Med. Publication Date: 1998 Mar |
Date Detail:
|
Created Date: 1998-04-07 Completed Date: 1998-04-07 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 9421642 Medline TA: Am J Respir Crit Care Med Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 938-49 Citation Subset: AIM; IM |
Affiliation:
|
Department of Medicine, Duke University Medical Center, Durham, North Carolina 27710, USA. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Acidosis
/
microbiology Animals Antibodies, Monoclonal / administration & dosage, therapeutic use* Blood Pressure Carbon Dioxide / blood Cardiac Output Cell Adhesion Molecules / immunology Chemotaxis, Leukocyte / immunology E-Selectin / immunology* Escherichia coli Infections / complications* Humans Hypertension, Pulmonary / microbiology Hypotension / microbiology Injections, Intravenous L-Selectin / immunology* Male Neutrophil Activation Neutrophils / immunology Oxygen / blood Papio Pulmonary Gas Exchange Respiration, Artificial Respiratory Distress Syndrome, Adult / microbiology, prevention & control* Sepsis / complications* Survival Rate Up-Regulation Urine Ventilation-Perfusion Ratio |
| Grant Support | |
ID/Acronym/Agency:
|
5PO1 HL42444-07/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Cell Adhesion Molecules; 0/E-Selectin; 124-38-9/Carbon Dioxide; 126880-86-2/L-Selectin; 7782-44-7/Oxygen |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Airway inflammation in young marijuana and tobacco smokers.
Next Document: Haemophilus influenzae in lung explants of patients with end-stage pulmonary disease.