| Antibody response of ewes and does to chimeric sheep-goat pregnancy. | |
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MedLine Citation:
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PMID: 7506943 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Antibody production was evaluated in 62 recipients of blastomere-aggregation sheep-goat embryos, including 23 multiparous ewes, 21 multiparous does, 16 primiparous does, and 2 virgin does. The reactivity of sera collected weekly after the embryo transfer surgery was compared to that of sera collected prior to the embryo transfer by means of 1) complement-dependent cytotoxicity tests against peripheral blood lymphocytes (PBLs) from the parents of the embryo(s) and from random-bred sheep and goats, 2) hemagglutination and hemolytic assays with red blood cells (RBCs) from the two sires of the embryo(s), and 3) assays with PBLs and RBCs following absorptions with RBCs and PBLs from the parents and offspring. Although cross-reactivity to ovine and caprine PBL antigens was present in the control sera of some recipients, xenogeneic immunization during pregnancy was detected in 20 of 30 recipients that experienced term pregnancy. The xenogeneic response involved the production of antibody that reacted with both PBLs and RBCs. Allogeneic responses to RBCs were not observed, but allogeneic responses to PBLs occurred frequently, beginning after the onset of the xenogeneic response in most recipients (98 +/- 28 vs. 57 +/- 15 days in ewes; 93 +/- 23 vs. 46 +/- 7 days in does; mean day of onset +/- SD). The onsets of the responses were examined in conjunction with data collected on fetal and placental chimerism to evaluate possible routes of immunization. The onsets of the allogeneic responses and the limited serum reactivity to third-party PBLs suggested that fetal lymphocytes leaking across the placenta immunized the recipients to parentally inherited polymorphic antigens. The xenogeneic responses were associated with placental chimerism and appeared to involve the recognition of a species-specific monomorphic antigen shared by PBLs and RBCs. Neither of the responses appeared to affect continuation of pregnancy to term. |
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Authors:
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N A Ruffing; G B Anderson; R H Bondurant; R L Pashen; D Bernoco |
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Publication Detail:
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Type: Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Biology of reproduction Volume: 49 ISSN: 0006-3363 ISO Abbreviation: Biol. Reprod. Publication Date: 1993 Dec |
Date Detail:
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Created Date: 1994-02-24 Completed Date: 1994-02-24 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 0207224 Medline TA: Biol Reprod Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 1260-9 Citation Subset: IM |
Affiliation:
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Department of Animal Science, University of California, Davis 95616. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Chimera / immunology* Cytotoxicity Tests, Immunologic Embryo Transfer Epitopes / immunology Erythrocytes / immunology Female Fetus / immunology Goats / immunology* Hemagglutination Tests Hemolysis Immune Sera / immunology Isoantibodies / blood Leukocytes / immunology Parity Placenta / immunology Pregnancy Pregnancy, Animal / immunology* Sheep / immunology* |
| Chemical | |
Reg. No./Substance:
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0/Epitopes; 0/Immune Sera; 0/Isoantibodies |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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