| Antibody-dependent cellular cytotoxicity mediated by polymorphonuclear leukocytes and mononuclear cells against HSV-1 infected primary cultures of rabbit corneal epithelium. | |
| | |
MedLine Citation:
|
PMID: 6386346 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
The roles of rabbit polymorphonuclear leukocytes (PMNL) and mononuclear cells (MC) for the regulation of ocular herpes simplex virus-1 (HSV-1) infection were studied. The antibody-dependent cellular cytotoxicity (ADCC) mediated by PMNL and MC from normal rabbit peripheral blood was assessed kinetically employing a specific 51Cr release assay. The HSV-1 infected primary cultures of rabbit corneal epithelium (PRCE) were used as the target cells to obtain a homologous assay system. The PRCE was prepared by an epithelium outgrowth technique and identified by electron microscopy. The expression of the surface HSV-1 antigens on PRCE was examined by indirect immunofluorescent staining; the cell population stained by fluorescein increased from 40% at 3 hr postinfection (PI) to 100% at 8 hr PI. To determine how early the cytotoxicity occurs, PRCE were infected with HSV-1 for 2 hrs. After 2 hrs, the ADCC was checked every 10 min for the first 40 min and then at 1, 2 and 4 hr of incubation. The cytotoxicity was apparent at 10 min postincubation and reached 46% by PMNL and 40% by MC at 4 hr postincubation (6 hr PI). Significant cytotoxic effect (26% by PMNL and 16% by MC) occurred as early as 3 hr PI. When the one-step growth cycle of HSV-1 was studied in the PRCE, HSV-1 had an eclipse period of 4 hr and a rise period of 8 hr. This suggests that rabbit PMNL and MC have the potential to eliminate the HSV-1 infected rabbit corneal epithelium before HSV matures in the cells. |
| | |
Authors:
|
J M Hill; B S Kwon; G L Colborn; Y Shimomura; L P Gangarosa |
Related Documents
:
|
6781356 - Theileriosis: cell-mediated and humoral immunity. 23212516 - Phase 1 trial of il-15 trans presentation blockade using humanized mik-beta-1 mab in pa... 696436 - Effect of dialysable leukocytes extracts on antibody-dependent cellular cytotoxicity an... 1673486 - Natural killer cell stimulatory factor (nksf) augments natural killer cell and antibody... 15924926 - Detection of antibodies to borrelia burgdorferi in naturally infected horses in the usa... 2452776 - Substances with affinity to a monoclonal aflatoxin b1 antibody in danish urine samples. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: Current eye research Volume: 3 ISSN: 0271-3683 ISO Abbreviation: Curr. Eye Res. Publication Date: 1984 Oct |
Date Detail:
|
Created Date: 1984-12-19 Completed Date: 1984-12-19 Revised Date: 2007-11-14 |
Medline Journal Info:
|
Nlm Unique ID: 8104312 Medline TA: Curr Eye Res Country: ENGLAND |
Other Details:
|
Languages: eng Pagination: 1203-12 Citation Subset: IM |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antibody-Dependent Cell Cytotoxicity* Antigens, Viral / immunology Cornea / immunology* Culture Techniques Epithelium / immunology Fluorescent Antibody Technique Immunity, Cellular Keratitis, Dendritic / immunology* Leukocytes / immunology* Microscopy, Electron Monocytes / immunology Neutrophils / immunology Rabbits |
| Grant Support | |
ID/Acronym/Agency:
|
DE-04917/DE/NIDCR NIH HHS; EY-04169/EY/NEI NIH HHS; EY-04636/EY/NEI NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antigens, Viral |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
Previous Document: Refractive keratoplasty: myopic keratomileusis in baboons.
Next Document: Association of alpha-crystallin with actin in cultured lens cells.