Document Detail

Antibody dependent cell-mediated cytotoxicity of Trypanosoma cruzi: the release of tritium-labelled RNA, DNA and protein.
MedLine Citation:
PMID:  351526     Owner:  NLM     Status:  MEDLINE    
The cytotoxicity of normal rat spleen cells to antibody-coated Trypanosoma cruzi epimastigotes has been studied by assaying the release of [3H]-labelled macromolecules from the parasites. The release of thymidine (DNA) is slower than the release of uridine (RNA), suggesting that the nucleus is broken down more slowly than the cytoplasmic membrane. Less than 50% of the leucine (protein) is released when the parasites are lysed, whereas uridine (RNA) is almost totally released. In practical terms these results show that the release of incorporated radioisotope-labelled uridine can be used as a sensitive assay for cytotoxicity of T. cruzi. Cytotoxicity by normal rat spleen cells is antibody dependent and proportional to the logarithm of effector cell number. The lag phase and the rate of RNA release is not altered by centrifuging the parasites and effector cells to enhance contacts between them.
C J Sanderson; M M Moreno; A F Lopez
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Parasitology     Volume:  76     ISSN:  0031-1820     ISO Abbreviation:  Parasitology     Publication Date:  1978 Jun 
Date Detail:
Created Date:  1978-08-14     Completed Date:  1978-08-14     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0401121     Medline TA:  Parasitology     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  299-307     Citation Subset:  IM    
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MeSH Terms
Antibody-Dependent Cell Cytotoxicity*
DNA / metabolism*
Leucine / metabolism
Proteins / metabolism*
RNA / metabolism*
Spleen / immunology
Thymidine / metabolism
Trypanosoma cruzi / immunology*,  metabolism
Uridine / metabolism
Reg. No./Substance:
0/Proteins; 50-89-5/Thymidine; 58-96-8/Uridine; 61-90-5/Leucine; 63231-63-0/RNA; 9007-49-2/DNA

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine

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