| Antibody-based nanoprobe for measurement of a fluorescent analyte in a single cell. | |
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MedLine Citation:
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PMID: 10888846 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We report here the application of an antibody-based nanoprobe for in situ measurements of a single cell. The nanoprobe employs antibody-based receptors targeted to a fluorescent analyte, benzopyrene tetrol (BPT), a metabolite of the carcinogen benzo[a]pyrene (BaP) and of the BaP-DNA adduct. Detection of BPT is of great biomedical interest, since this species can serve as a biomarker for monitoring DNA damage due to BaP exposure and for possible precancer diagnosis. The measurements were performed on the rat liver epithelial clone 9 cell line, which was used as the model cell system. Before making measurements, the cells were treated with BPT. Nanoprobes were inserted into individual cells, incubated 5 min to allow antigen-antibody binding, and then removed for fluorescence detection. We determined a concentration of 9.6+/-0.2x10(-11) M for BPT in the individual cells investigated. The results demonstrate the possibility of in situ measurements inside a single cell using an antibody-based nanoprobe. |
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Authors:
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T Vo-Dinh; J P Alarie; B M Cullum; G D Griffin |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, Non-P.H.S. |
Journal Detail:
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Title: Nature biotechnology Volume: 18 ISSN: 1087-0156 ISO Abbreviation: Nat. Biotechnol. Publication Date: 2000 Jul |
Date Detail:
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Created Date: 2000-10-05 Completed Date: 2000-10-05 Revised Date: 2006-11-15 |
Medline Journal Info:
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Nlm Unique ID: 9604648 Medline TA: Nat Biotechnol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 764-7 Citation Subset: IM |
Affiliation:
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Advanced Monitoring Development Group, Life Sciences Division, P.O. Box 2008, Oak Ridge National Laboratory, Oak Ridge TN 37831-6101, USA. |
Export Citation:
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| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies / immunology* Benzo(a)pyrene / metabolism Biological Markers Cell Line DNA Adducts / metabolism Epithelium / metabolism Fluorescent Antibody Technique / instrumentation* Liver / metabolism Microscopy, Electron, Scanning Pyrenes / metabolism Rats Spectrometry, Fluorescence / instrumentation*, methods* |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Biological Markers; 0/DNA Adducts; 0/Pyrenes; 0/benzo(a)pyrene tetrol; 50-32-8/Benzo(a)pyrene |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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