Document Detail


Antibody-aptamer functionalized fibre-optic biosensor for specific detection of Listeria monocytogenes from food.
MedLine Citation:
PMID:  20337767     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
AIM: To develop antibody-aptamer functionalized fibre-optic biosensor for specific detection of Listeria monocytogenes from food products.
METHODS AND RESULTS: Aptamer, a single-stranded oligonucleotide ligand that displays affinity for the target molecule, was used in the assay to provide sensor specificity. Aptamer-A8, specific for internalin A, an invasive protein of L. monocytogenes, was used in the fibre-optic sensor together with antibody in a sandwich format for detection of L. monocytogenes from food. Biotinylated polyclonal anti-Listeria antibody, P66, was immobilized on streptavidin-coated optical waveguide surface for capturing bacteria, and Alexa Fluor 647-conjugated A8 was used as a reporter. The biosensor was able to selectively detect pathogenic Listeria in pure culture and in mixture with other bacteria at a concentration of approx. 10(3) CFU ml(-1). This sensor also successfully detected L. monocytogenes cells from artificially contaminated (initial inoculation of 10(2) CFU 25 g(-1) ) ready-to-eat meat products such as sliced beef, chicken and turkey after 18 h of enrichment.
CONCLUSION: Based on the data presented in this study, the antibody-aptamer functionalized fibre-optic biosensor could be used as a detection tool for sensitive and specific detection of L. monocytogenes from foods.
SIGNIFICANCE AND IMPACT OF THE STUDY: The study demonstrates feasibility and novel application of aptamer on fibre-optic biosensor platform for the sensitive detection of L. monocytogenes from food products.
Authors:
S H Ohk; O K Koo; T Sen; C M Yamamoto; A K Bhunia
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of applied microbiology     Volume:  109     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-11-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  808-17     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. Journal compilation © 2010 The Society for Applied Microbiology.
Affiliation:
Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN 47907-2009, USA.
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