Document Detail


Antibody-aptamer functionalized fibre-optic biosensor for specific detection of Listeria monocytogenes from food.
MedLine Citation:
PMID:  20337767     Owner:  NLM     Status:  In-Process    
Abstract/OtherAbstract:
AIM: To develop antibody-aptamer functionalized fibre-optic biosensor for specific detection of Listeria monocytogenes from food products.
METHODS AND RESULTS: Aptamer, a single-stranded oligonucleotide ligand that displays affinity for the target molecule, was used in the assay to provide sensor specificity. Aptamer-A8, specific for internalin A, an invasive protein of L. monocytogenes, was used in the fibre-optic sensor together with antibody in a sandwich format for detection of L. monocytogenes from food. Biotinylated polyclonal anti-Listeria antibody, P66, was immobilized on streptavidin-coated optical waveguide surface for capturing bacteria, and Alexa Fluor 647-conjugated A8 was used as a reporter. The biosensor was able to selectively detect pathogenic Listeria in pure culture and in mixture with other bacteria at a concentration of approx. 10(3) CFU ml(-1). This sensor also successfully detected L. monocytogenes cells from artificially contaminated (initial inoculation of 10(2) CFU 25 g(-1) ) ready-to-eat meat products such as sliced beef, chicken and turkey after 18 h of enrichment.
CONCLUSION: Based on the data presented in this study, the antibody-aptamer functionalized fibre-optic biosensor could be used as a detection tool for sensitive and specific detection of L. monocytogenes from foods.
SIGNIFICANCE AND IMPACT OF THE STUDY: The study demonstrates feasibility and novel application of aptamer on fibre-optic biosensor platform for the sensitive detection of L. monocytogenes from food products.
Authors:
S H Ohk; O K Koo; T Sen; C M Yamamoto; A K Bhunia
Related Documents :
12648837 - History and epidemiology of listeriosis.
18395697 - Effects of commonly used food preservatives on biofilm formation of streptococcus mutan...
8429537 - Genetic characterisation of isolates of listeria monocytogenes from man, animals and food.
16865897 - Consumer knowledge, storage, and handling practices regarding listeria in frankfurters ...
8694027 - Chronic low intakes of vitamin a-rich foods in households with xerophthalmic children: ...
16300427 - Combined dieting and stress evoke exaggerated responses to opioids in binge-eating rats.
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't; Research Support, U.S. Gov't, Non-P.H.S.    
Journal Detail:
Title:  Journal of applied microbiology     Volume:  109     ISSN:  1365-2672     ISO Abbreviation:  J. Appl. Microbiol.     Publication Date:  2010 Sep 
Date Detail:
Created Date:  2010-11-05     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  9706280     Medline TA:  J Appl Microbiol     Country:  England    
Other Details:
Languages:  eng     Pagination:  808-17     Citation Subset:  IM    
Copyright Information:
© 2010 The Authors. Journal compilation © 2010 The Society for Applied Microbiology.
Affiliation:
Molecular Food Microbiology Laboratory, Department of Food Science, Purdue University, West Lafayette, IN 47907-2009, USA.
Export Citation:
APA/MLA Format     Download EndNote     Download BibTex
MeSH Terms
Descriptor/Qualifier:

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


Previous Document:  smcL as a novel diagnostic marker for quantitative detection of Listeria ivanovii in biological samp...
Next Document:  Cutaneous intravascular anaplastic large cell lymphoma.