Document Detail

Antibody against single-stranded DNA detects both programmed cell death and drug-induced apoptosis.
MedLine Citation:
PMID:  7517929     Owner:  NLM     Status:  MEDLINE    
Cyclophosphamide induced fragmented nuclei in mouse thymic epithelial cells. Agarose gel electrophoresis showed the fragmentation of the DNA extracted from mouse thymus exposed to cyclophosphamide. The cell death induced by cyclophosphamide was considered to be apoptotic. Polyclonal antibody against single-stranded DNA was used immunohistochemically to detect apoptotic cell death in thymic epithelial cells. This antibody also detected programmed cell death in the interdigital necrotic zone of the mouse limb plate on day 14 of gestation, and in the ganglion of the trigeminal nerve on day 13 of gestation. These results show that the antibody specific for single-stranded DNA detected both drug-induced apoptosis and programmed cell death during embryogenesis.
I Naruse; H Keino; Y Kawarada
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Histochemistry     Volume:  101     ISSN:  0301-5564     ISO Abbreviation:  Histochemistry     Publication Date:  1994 Jan 
Date Detail:
Created Date:  1994-08-11     Completed Date:  1994-08-11     Revised Date:  2003-11-14    
Medline Journal Info:
Nlm Unique ID:  0411300     Medline TA:  Histochemistry     Country:  GERMANY    
Other Details:
Languages:  eng     Pagination:  73-8     Citation Subset:  IM    
Department of Morphology, Institute for Developmental Research, Aichi Prefectural Colony, Japan.
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MeSH Terms
Apoptosis / drug effects*,  physiology*
Cell Death / drug effects
Cyclophosphamide / toxicity
DNA, Single-Stranded / analysis*,  immunology
Dendritic Cells / chemistry,  cytology,  pathology
Eosine Yellowish-(YS)
Extremities / embryology
Mice, Inbred ICR
Staining and Labeling / methods
Trigeminal Ganglion / chemistry,  cytology*,  embryology
Reg. No./Substance:
0/Antibodies; 0/DNA, Single-Stranded; 17372-87-1/Eosine Yellowish-(YS); 50-18-0/Cyclophosphamide; 517-28-2/Hematoxylin

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