| Antibody against single-stranded DNA detects both programmed cell death and drug-induced apoptosis. | |
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MedLine Citation:
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PMID: 7517929 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Cyclophosphamide induced fragmented nuclei in mouse thymic epithelial cells. Agarose gel electrophoresis showed the fragmentation of the DNA extracted from mouse thymus exposed to cyclophosphamide. The cell death induced by cyclophosphamide was considered to be apoptotic. Polyclonal antibody against single-stranded DNA was used immunohistochemically to detect apoptotic cell death in thymic epithelial cells. This antibody also detected programmed cell death in the interdigital necrotic zone of the mouse limb plate on day 14 of gestation, and in the ganglion of the trigeminal nerve on day 13 of gestation. These results show that the antibody specific for single-stranded DNA detected both drug-induced apoptosis and programmed cell death during embryogenesis. |
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Authors:
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I Naruse; H Keino; Y Kawarada |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Histochemistry Volume: 101 ISSN: 0301-5564 ISO Abbreviation: Histochemistry Publication Date: 1994 Jan |
Date Detail:
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Created Date: 1994-08-11 Completed Date: 1994-08-11 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 0411300 Medline TA: Histochemistry Country: GERMANY |
Other Details:
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Languages: eng Pagination: 73-8 Citation Subset: IM |
Affiliation:
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Department of Morphology, Institute for Developmental Research, Aichi Prefectural Colony, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies* Apoptosis / drug effects*, physiology* Cell Death / drug effects Cyclophosphamide / toxicity DNA, Single-Stranded / analysis*, immunology Dendritic Cells / chemistry, cytology, pathology Eosine Yellowish-(YS) Extremities / embryology Female Hematoxylin Immunohistochemistry Male Mice Mice, Inbred ICR Necrosis Pregnancy Staining and Labeling / methods Trigeminal Ganglion / chemistry, cytology*, embryology |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/DNA, Single-Stranded; 17372-87-1/Eosine Yellowish-(YS); 50-18-0/Cyclophosphamide; 517-28-2/Hematoxylin |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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