| Antibody action after phagocytosis promotes Cryptococcus neoformans and Cryptococcus gattii macrophage exocytosis with biofilm-like microcolony formation. | |
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MedLine Citation:
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PMID: 18384661 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Antibody-mediated phagocytosis was discovered over a century ago but little is known about antibody effects in phagolysosomes. We explored the consequences of antibody-mediated phagocytosis for two closely related human pathogenic fungal species, Cryptococcus neoformans and Cryptococcus gattii, of which C. neoformans encompasses two varieties: neoformans and grubii. The interaction between C. neoformans varieties grubii and neoformans and host cells has been extensively studied, but that of C. gattii and macrophages remains largely unexplored. Like C. neoformans, antibody-mediated phagocytosis of C. gattii cells was followed by intracellular replication, host cell cytoplasmic polysaccharide accumulation and phagosomal extrusion. Both C. gattii and C. neoformans cells exited macrophages in biofilm-like microcolonies where the yeast cells were aggregated in a polysaccharide matrix that contained bound antibody. In contrast, complement-opsonized C. neoformans variety grubii cells were released from macrophages dispersed as individual cells. Hence, both antibody- and complement-mediated phagocytosis resulted in intracellular replication but the mode of opsonization affected the outcome of exocytosis. The biofilm-like microcolony exit strategy of C. neoformans and C. gattii following antibody opsonization reduced fungal cell dispersion. This finding suggests that antibody agglutination effects persist in the phagosome to entangle nascent daughter cells and this phenomenon may contribute to antibody-mediated protection. |
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Authors:
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Mauricio Alvarez; Carolyn Saylor; Arturo Casadevall |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2008-04-01 |
Journal Detail:
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Title: Cellular microbiology Volume: 10 ISSN: 1462-5822 ISO Abbreviation: Cell. Microbiol. Publication Date: 2008 Aug |
Date Detail:
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Created Date: 2008-08-20 Completed Date: 2008-09-24 Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 100883691 Medline TA: Cell Microbiol Country: England |
Other Details:
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Languages: eng Pagination: 1622-33 Citation Subset: IM |
Affiliation:
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Department of Medicine, Albert Einstein College of Medicine, 1300 Morris Park Ave., Bronx, NY 10461, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antibodies Biofilms Cell Line Cells, Cultured Cryptococcosis / immunology* Cryptococcus / immunology* Cryptococcus neoformans / immunology* Exocytosis* Macrophages, Alveolar / immunology, microbiology* Macrophages, Peritoneal / immunology, microbiology* Mice Phagocytosis Phagosomes / microbiology |
| Grant Support | |
ID/Acronym/Agency:
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AI033142-11/AI/NIAID NIH HHS; AI033774-11/AI/NIAID NIH HHS; HL059842-08/HL/NHLBI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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