Document Detail


Antibodies to prothrombin crossreact with plasminogen in patients developing myocardial infarction.
MedLine Citation:
PMID:  9488629     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
In a prospective study on healthy middle-aged men, high level of antibodies to prothrombin implied a risk of myocardial infarction. The possible mechanism(s) of these antibodies in coronary thrombosis are not known. Because prothrombin belongs to the kringle proteins and shares structural homology with a fibrinolytic kringle protein plasminogen, we studied whether antibodies to prothrombin crossreact with plasminogen. Sera from 17 healthy middle-aged men who later developed myocardial infarction were studied. Binding of antibodies to immobilized prothrombin (EIA) was inhibited by using soluble prothrombin, plasminogen and synthetic peptides of 20 amino acids from plasminogen kringle 5 (P304, P305) and from prothrombin kringle 2 (P302) as inhibitors. The peptides contained the conserved pentapeptide CRNPD of the kringle proteins. Soluble prothrombin inhibited up to 50% the binding of antibodies to immobilized prothrombin in all sera. Plasminogen inhibited binding in 9/17 (53%) sera (a decrease of at least 20%). P305 inhibited binding to prothrombin in 8/17 (47%), P304 in 4/17 (23%) and P302 in 6/17 (35%) sera. In structural analysis, presentation of the pentapeptide was conformationally different between the peptides. We conclude that crossreactive antibodies binding to prothrombin and plasminogen occur in sera of patients later developing myocardial infarction. The crossreactive epitope seems to be conformational and include the conserved pentapeptide of the kringle proteins. These antibodies may interfere with the fibrinolytic function of plasminogen and contribute to the development of myocardial infarction.
Authors:
M Puurunen; M Mänttäri; V Manninen; T Palosuo; O Vaarala
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Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't    
Journal Detail:
Title:  British journal of haematology     Volume:  100     ISSN:  0007-1048     ISO Abbreviation:  Br. J. Haematol.     Publication Date:  1998 Feb 
Date Detail:
Created Date:  1998-03-10     Completed Date:  1998-03-10     Revised Date:  2006-11-15    
Medline Journal Info:
Nlm Unique ID:  0372544     Medline TA:  Br J Haematol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  374-9     Citation Subset:  IM    
Affiliation:
Department of Biochemistry, National Public Health Institute, Helsinki, Finland.
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MeSH Terms
Descriptor/Qualifier:
Antibodies / immunology*
Cross Reactions
Enzyme-Linked Immunosorbent Assay
Humans
Immunoglobulin G / immunology
Male
Middle Aged
Myocardial Infarction / immunology*
Plasminogen / immunology*
Prospective Studies
Prothrombin / immunology*
Chemical
Reg. No./Substance:
0/Antibodies; 0/Immunoglobulin G; 9001-26-7/Prothrombin; 9001-91-6/Plasminogen

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