| Antibodies to intercellular adhesion molecule 1/lymphocyte function-associated antigen 1 prevent crescent formation in rat autoimmune glomerulonephritis. | |
| | |
| Jump to Full Text | |
MedLine Citation:
|
PMID: 8094735 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
|
In patients with glomerulonephritis widespread crescents are associated with a poor prognosis. Crescent formation appears to depend on the migration of mononuclear cells into Bowman's space, and therefore the interaction between leukocytes and glomerular endothelium may be a critical event in the genesis of crescents. We performed the present study to determine the effects of mouse monoclonal antibodies to the adhesion molecules intercellular adhesion molecule 1 (ICAM-1) and lymphocyte function-associated antigen 1 (LFA-1) in a model of crescentic glomerulonephritis in Wistar-Kyoto rats, induced by immunization with bovine glomerular basement membrane (GBM). By 10-14 d after immunization, the rats had developed circulating anti-GBM antibodies, reactive with the alpha 3 chain of type IV collagen (the Goodpasture antigen), accompanied by proteinuria, accumulation of rat immunoglobulin (Ig)G in the GBM, increased expression of ICAM-1 by glomerular endothelial cells, infiltration of glomerular tufts with LFA-1+ T cells and monocyte/macrophages, and early crescents. At 5 wk all rats had diffuse fibrocellular crescents, glomerular sclerosis, and tubulointerstitial damage. All rats developed severe renal insufficiency and died by 5 or 6 wk. The administration of monoclonal antibodies to rat ICAM-1 and LFA-1 markedly decreased the severity of the renal disease. In a group of rats injected three times a week with the monoclonal antibodies, from 2 d before immunization with GBM to day 14, glomerular abnormalities and proteinuria were virtually absent at day 14; even at 5 wk glomerular disease was quite mild, with only slight crescent formation and with only a mild decrease in renal function. When treatment was continued until 5 wk, the beneficial effects were even more marked, with virtual absence of crescents and with preservation of normal renal function. In a group of rats in which treatment was initiated on day 14, shortly after the appearance of glomerular abnormalities, progression of the disease was appreciably retarded, and the decrease in renal function was inhibited. The kidneys of rats treated from days -2 to 14 with antibodies to ICAM-1 and LFA-1 showed bright linear staining for rat IgG along the GBM, which did not differ in intensity from that seen in untreated rats. Furthermore, the titers of anti-GBM antibodies at 2 wk in treated rats were not lower than that seen in most of the untreated rats. There was, however, moderate reduction of anti-GBM antibodies at 5 wk in the treated rats.(ABSTRACT TRUNCATED AT 400 WORDS) |
| | |
Authors:
|
K Nishikawa; Y J Guo; M Miyasaka; T Tamatani; A B Collins; M S Sy; R T McCluskey; G Andres |
Related Documents
:
|
983705 - Participation of blood born cells in rat masugi nephritis. 1563095 - Induction of autoantibodies to rat corneal protein 54. 21161045 - Activation of amp deaminase and adenosine deaminase in the liver during ammonia poisoni... 6527375 - Experimental autoimmune glomerulonephritis with pulmonary hemorrhage in rats. the dose-... 983705 - Participation of blood born cells in rat masugi nephritis. 16845685 - C-peptide improves neuropathy in type 1 diabetic bb/wor-rats. |
Publication Detail:
|
Type: Journal Article; Research Support, U.S. Gov't, P.H.S. |
Journal Detail:
|
Title: The Journal of experimental medicine Volume: 177 ISSN: 0022-1007 ISO Abbreviation: J. Exp. Med. Publication Date: 1993 Mar |
Date Detail:
|
Created Date: 1993-03-19 Completed Date: 1993-03-19 Revised Date: 2009-11-18 |
Medline Journal Info:
|
Nlm Unique ID: 2985109R Medline TA: J Exp Med Country: UNITED STATES |
Other Details:
|
Languages: eng Pagination: 667-77 Citation Subset: IM |
Affiliation:
|
Department of Pathology, Massachusetts General Hospital, Boston. |
Export Citation:
|
APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
|
Animals Antibodies, Monoclonal / immunology, pharmacology* Autoimmune Diseases / pathology, physiopathology, prevention & control* Basement Membrane / immunology, pathology Cell Adhesion Molecules / analysis, immunology*, metabolism Endothelium / chemistry, immunology, pathology Fluorescent Antibody Technique Glomerulonephritis / pathology, physiopathology, prevention & control* Immunoglobulin G / analysis, metabolism Immunohistochemistry Intercellular Adhesion Molecule-1 Kidney Glomerulus / chemistry, immunology, pathology Leukocytes / chemistry, immunology, pathology Lymphocyte Function-Associated Antigen-1 / analysis, immunology*, metabolism Macrophages / immunology, pathology Rats Rats, Inbred WKY T-Lymphocytes / immunology, pathology |
| Grant Support | |
ID/Acronym/Agency:
|
DK-36807/DK/NIDDK NIH HHS |
| Chemical | |
Reg. No./Substance:
|
0/Antibodies, Monoclonal; 0/Cell Adhesion Molecules; 0/Immunoglobulin G; 0/Lymphocyte Function-Associated Antigen-1; 126547-89-5/Intercellular Adhesion Molecule-1 |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
| Full Text | |
|
Journal Information Journal ID (nlm-ta): J Exp Med ISSN: 0022-1007 ISSN: 1540-9538 Publisher: The Rockefeller University Press |
Article Information Download PDF  Print publication date: Day: 1 Month: 3 Year: 1993 Volume: 177 Issue: 3 First Page: 667 Last Page: 677 ID: 2190927 Publisher Id: 93171807 PubMed Id: 8094735 |
| Antibodies to intercellular adhesion molecule 1/lymphocyte function- associated antigen 1 prevent crescent formation in rat autoimmune glomerulonephritis | |
Article Categories:
|
|
Previous Document: Evidence that the T cell repertoire of normal rats contains cells with the potential to cause diabet...
Next Document: Abnormal in vitro proliferation and differentiation of T cell colony-forming cells in patients with ...