Document Detail


Antibodies to decorin-binding protein B (DbpB) in the diagnosis of Lyme neuroborreliosis in children.
MedLine Citation:
PMID:  25316329     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
BACKGROUND: Laboratory support is needed to confirm the clinical diagnosis of Lyme neuroborreliosis (LNB). Antibodies to Borrelia-specific proteins have been used to improve serological diagnostics. The aims of this study were to assess the occurrence of antibodies to decorin-binding protein B (DbpB) in serum and cerebrospinal fluid (CSF) in children with LNB and to evaluate the performance of DbpB variants in the diagnosis of LNB in children.
METHODS: Serum and CSF sample pairs were available for 57 children evaluated for LNB. Based on the presence of anti-flagella antibodies and pleocytosis in the CSF, patients were divided into three different groups: confirmed LNB (n=24), possible LNB (n=16), and non LNB (n=17). Recombinant DbpBs from three Borrelia burgdorferi sensu lato species-Borrelia burgdorferi sensu stricto, Borrelia garinii, and Borrelia afzelii-were used in an ELISA to detect IgG antibodies.
RESULTS: The sensitivity of variant recombinant DbpBs in serum and CSF samples varied between 0% and 46% and between 0% and 42%, respectively. In CSF, the most sensitive antigen was the DbpB variant from B. garinii.
CONCLUSIONS: Serum or CSF antibodies to DbpB do not appear to be beneficial in the laboratory diagnosis of LNB in children.
Authors:
Heidi Sillanpää; Barbro H Skogman; Heikki Sarvas; Ilkka J T Seppälä; Pekka Lahdenne
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2014-10-11
Journal Detail:
Title:  International journal of infectious diseases : IJID : official publication of the International Society for Infectious Diseases     Volume:  -     ISSN:  1878-3511     ISO Abbreviation:  Int. J. Infect. Dis.     Publication Date:  2014 Oct 
Date Detail:
Created Date:  2014-10-15     Completed Date:  -     Revised Date:  2014-10-16    
Medline Journal Info:
Nlm Unique ID:  9610933     Medline TA:  Int J Infect Dis     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Copyright Information:
Copyright © 2014. Published by Elsevier Ltd.
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