| Antibodies to Wheat High-Molecular-Weight Glutenin Subunits in Patients with Celiac Disease. | |
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MedLine Citation:
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PMID: 22813868 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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Background: Wheat gluten comprises gliadins and glutenins. The high-molecular-weight (HMW) glutenin subunits (GS)-1Dy10 are toxic for patients with celiac disease (CD). This study aimed to assess whether CD patients mount a serological response to HMW-GS-1Dy10. Methods: Recombinant HMW-GS-1Dy10 was deamidated using human recombinant tissue transglutaminase. MALDI-TOF was performed to compare the level of deamidation of glutamine residues between material before and after treatment. Enzyme-linked immunosorbent assays were developed. Sera from patients with untreated CD and gastrointestinal disease controls were tested and receiver operator characteristics were used to calculate cutoffs. Results: MALDI-TOF revealed a number of fragments matching known HMW-GS-1Dy10 sequences within both the deamidated and non-deamidated material. Evidence of deamidation of glutamine residues was found only within the human transglutaminase-treated material. Patients with untreated CD had significantly increased levels of serum antibodies to HMW-GS-1Dy10 compared to controls. Undeamidated HMW-GS-1Dy10 IgA antibodies had sensitivities and specificities of 72.5 and 78.26%, respectively. Deamidated HMW-GS-1Dy10 IgA antibodies had sensitivities and specificities of 76.8 and 65.2%. Undeamidated HMW-GS-1Dy10 IgG antibodies had sensitivities and specificities of 75.3 and 68.1%. Deamidated HMW-GS-1Dy10 IgG antibodies had sensitivities and specificities of 36.2 and 92.8%. Conclusion: Patients with untreated CD have raised antibody levels to HMW-GS-1Dy10, indicating the participation of these proteins in the adaptive immune response to gluten. Discrimination between CD patients and controls is not enhanced by deamidation of HMW-GS-1Dy10. Thus antibodies to these proteins are not useful markers for CD detection. |
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Authors:
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H Julia Ellis; Pablo Lozano-Sanchez; Carmen Bermudo Redondo; Tanja Suligoj; Federico Biagi; Paola I Bianchi; Gino R Corazza; Annalisa De Silvestri; Enzo Bravi; Ioanis Katakis; Ciara K O'Sullivan; Paul J Ciclitira |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-7-17 |
Journal Detail:
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Title: International archives of allergy and immunology Volume: 159 ISSN: 1423-0097 ISO Abbreviation: - Publication Date: 2012 Jul |
Date Detail:
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Created Date: 2012-7-20 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 9211652 Medline TA: Int Arch Allergy Immunol Country: - |
Other Details:
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Languages: ENG Pagination: 428-434 Citation Subset: - |
Copyright Information:
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Copyright © 2012 S. Karger AG, Basel. |
Affiliation:
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Division of Diabetes and Nutritional Sciences, King's College London, London, UK. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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