Document Detail


Antibodies reactive with human immunodeficiency virus gag-coded antigens (gag reactive only) are a major cause of enzyme-linked immunosorbent assay reactivity in a blood donor population.
MedLine Citation:
PMID:  3259246     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Normal blood donors were examined for human immunodeficiency virus (HIV)-reactive antibodies with both virus- and Escherichia coli-expressed env- and gag-coded antigens. The frequency of samples from normal (low-risk) donors that were repeatedly reactive with an HIV enzyme-linked immunosorbent assay blood screening test (Du Pont Co.) was 0.6%. Two classes of HIV serological reactivity were identified: a minor env-reactive class (0.03 to 0.06% of donors) and the predominant env-nonreactive gag-reactive class (gag reactive only [GRO]) (0.4 to 0.5% of donors). Assignment of env reactivity was made by a synthetic (recombinant) env enzyme-linked immunosorbent assay and virus immunoblot. Most GRO sera reacted with p15/p17 bands on HIV immunoblot. Antibody specificity in GRO sera was confirmed by competition-binding studies with viral gag and E. coli-expressed p55gag. This study provides independent verification that gag-specific antibodies are present in many env-nonreactive sera. More serological and virological studies of individuals with this antibody pattern should be pursued to determine the origin of these gag-reactive antibodies.
Authors:
D E Tribe; D L Reed; P Lindell; W R Kenealy; B Q Ferguson; R Cybulski; D Winslow; D M Waselefsky; S R Petteway
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Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Journal of clinical microbiology     Volume:  26     ISSN:  0095-1137     ISO Abbreviation:  J. Clin. Microbiol.     Publication Date:  1988 Apr 
Date Detail:
Created Date:  1988-06-16     Completed Date:  1988-06-16     Revised Date:  2009-11-18    
Medline Journal Info:
Nlm Unique ID:  7505564     Medline TA:  J Clin Microbiol     Country:  UNITED STATES    
Other Details:
Languages:  eng     Pagination:  641-7     Citation Subset:  IM; X    
Affiliation:
Medical Products Department, E. I. du Pont de Nemours and Company, Wilmington, Delaware 19898.
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MeSH Terms
Descriptor/Qualifier:
Antibodies, Viral / analysis*
Antibody Specificity
Antigens, Viral / immunology*
Blood Donors*
Enzyme-Linked Immunosorbent Assay
Gene Products, gag
HIV / immunology*
HIV Antibodies
HIV Antigens
Humans
Immunoassay
Recombinant Proteins / immunology
Retroviridae Proteins / immunology*
Chemical
Reg. No./Substance:
0/Antibodies, Viral; 0/Antigens, Viral; 0/Gene Products, gag; 0/HIV Antibodies; 0/HIV Antigens; 0/Recombinant Proteins; 0/Retroviridae Proteins
Comments/Corrections

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