| Antibodies immobilized as arrays to profile protein post-translational modifications in mammalian cells. | |
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MedLine Citation:
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PMID: 15123764 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previously, we demonstrated that antibodies printed on a solid support were able to detect protein-protein interaction in mammalian cells. Here we further developed the antibody array system for detecting proteins with various post-translational modifications in mammalian cells. In this novel approach, immunoprecipitated proteins were labeled with fluorescent dye followed by incubation over antibody arrays. Targeted proteins, captured by the antibodies immobilized on PVDF membrane or glass slide, were detected by means of near infrared fluorescent scanner or fluorescent microscopy. To demonstrate the application of the antibody arrays in protein post-translational modifications, we profiled protein tyrosine phosphorylation, ubiquitination, and acetylation in mammalian cells under different conditions. Our results indicate that antibody array technology can provide a powerful means of profiling a large number of proteins with different post-translational modifications in cells. |
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Authors:
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Stanimir S Ivanov; Alicia S Chung; Zheng-long Yuan; Ying-jie Guan; Katherine V Sachs; Jonathan S Reichner; Y Eugene Chin |
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Publication Detail:
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Type: Journal Article; Research Support, U.S. Gov't, P.H.S. Date: 2004-05-03 |
Journal Detail:
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Title: Molecular & cellular proteomics : MCP Volume: 3 ISSN: 1535-9476 ISO Abbreviation: Mol. Cell Proteomics Publication Date: 2004 Aug |
Date Detail:
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Created Date: 2004-08-16 Completed Date: 2005-03-11 Revised Date: 2007-11-14 |
Medline Journal Info:
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Nlm Unique ID: 101125647 Medline TA: Mol Cell Proteomics Country: United States |
Other Details:
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Languages: eng Pagination: 788-95 Citation Subset: IM |
Affiliation:
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Department of Pathology & Laboratory Medicine, Brown University Medical School, Providence, RI 02912, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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3T3 Cells Acetylation Animals Antibodies* Cell Line Fluorescent Antibody Technique Fluorescent Dyes Gene Expression Profiling Hela Cells Humans Mice Phosphorylation Protein Array Analysis* Protein Processing, Post-Translational* Tyrosine / metabolism* Ubiquitin / metabolism* |
| Grant Support | |
ID/Acronym/Agency:
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GM-66194/GM/NIGMS NIH HHS; R01 CA-82549/CA/NCI NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Antibodies; 0/Fluorescent Dyes; 0/Ubiquitin; 55520-40-6/Tyrosine |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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