Document Detail


Antibiotic susceptibility of potentially probiotic Bifidobacterium isolates from the human gastrointestinal tract.
MedLine Citation:
PMID:  9674160     Owner:  NLM     Status:  MEDLINE    
Abstract/OtherAbstract:
Sixteen Bifidobacterium isolates from the human gastrointestinal tract were assayed for susceptibility to 44 antibiotics by soft agar overlay disc diffusion on TPY agar. Five isolates (3/7 B. bifidum and 2/3 B. breve) exhibited atypical antibiotic susceptibility profiles. Poor growth in the agar overlay accounted for susceptibility of B. bifidum but not B. breve isolates. All other isolates were resistant to cefoxitin (30 micrograms), aztreonam (30 micrograms), vancomycin (30 micrograms), amikacin (30 micrograms), gentamicin (10 micrograms), kanamycin (30 micrograms), streptomycin (10 micrograms), fusidic acid (10 micrograms), trimethoprim (5 micrograms), norfloxacin (10 micrograms), nalidixic acid (30 micrograms), metronidazole (5 micrograms), polymyxin B (300 micrograms) and colistin sulphate (10 micrograms), and they were susceptible to the six penicillins studied, cephalothin (30 micrograms), cefuroxime (30 micrograms), cefaclor (30 micrograms), ceftizoxime (30 micrograms), cefotaxime (30 micrograms), bacitracin (10 micrograms), chloramphenicol (30 micrograms), erythromycin (15 micrograms), clindamycin (2 micrograms), rifampicin (5 micrograms) and nitrofurantoin (300 micrograms). In addition, they varied in their susceptibility to cephradine (30 micrograms), cephazolin (30 micrograms), cefoperazone (75 micrograms), ceftriaxone (30 micrograms), ofloxacin (5 micrograms) and furazolidone (15 micrograms). They were resistant, or only marginally moderately susceptible, to ceftazidime (30 micrograms), netilmicin (10 micrograms), sulphamethoxazole (100 micrograms), cotrimoxazole (25 micrograms) and ciprofloxacin (5 micrograms), and susceptible or marginally moderately susceptible to tetracycline (30 micrograms). All B. bifidum isolates were susceptible to cefixime (5 micrograms). Four microorganism-drug combinations were evaluated for beta-lactamase activity but its absence suggested that cell wall impermeability was responsible for cephalosporin resistance among bifidobacteria. The antibiotic susceptibility of B. animalis 25527T was similar to that of the human isolates.
Authors:
W P Charteris; P M Kelly; L Morelli; J K Collins
Publication Detail:
Type:  Journal Article    
Journal Detail:
Title:  Letters in applied microbiology     Volume:  26     ISSN:  0266-8254     ISO Abbreviation:  Lett. Appl. Microbiol.     Publication Date:  1998 May 
Date Detail:
Created Date:  1998-08-14     Completed Date:  1998-08-14     Revised Date:  2006-04-13    
Medline Journal Info:
Nlm Unique ID:  8510094     Medline TA:  Lett Appl Microbiol     Country:  ENGLAND    
Other Details:
Languages:  eng     Pagination:  333-7     Citation Subset:  IM    
Affiliation:
SET Consultants Ltd, Douglas, Cork, Ireland. bcharteris@awg.ie
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MeSH Terms
Descriptor/Qualifier:
Anti-Bacterial Agents / pharmacology*
Bifidobacterium / drug effects*,  isolation & purification
Digestive System / microbiology*
Drug Resistance, Microbial
Feces / microbiology
Humans
Microbial Sensitivity Tests
Probiotics*
beta-Lactamases / metabolism
Chemical
Reg. No./Substance:
0/Anti-Bacterial Agents; EC 3.5.2.6/beta-Lactamases

From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine


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