| Antibacterial resistance induced by recombinant interleukin 1 in myelosuppressed mice: effect of treatment schedule and correlation with colony-stimulating activity in the bloodstream. | |
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MedLine Citation:
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PMID: 2111738 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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We have investigated the effects of interleukin 1 (IL-1) administration on the ability of neutropenic mice to resist Pseudomonas aeruginosa challenge in vivo. Cyclophosphamide-treated mice received human rIL-1 beta at 7.0, 0.7, or 00.7 micrograms/kg, according to different regimens, to be challenged with a lethal ip inoculum of pseudomonas cells 5 days after myelosuppression. The repeated exposure of the neutropenic mice to an overall cytokine dosage of 7.0 or 0.7 micrograms/kg during the 4 days after myelosuppression was found to optimally restore the animals' antibacterial resistance. However, when administered as a single injection 24 hr before challenge, the same dosages of IL-1 had lower or no effect in enhancing survival, primarily leading only to a reduction in the amount of antipseudomonal chemotherapy required for cure. The regimen of IL-1 administration conferring optimal protection also resulted in a decrease in the number of pseudomonas cells recovered from the peritoneal cavity of infected mice. This regimen accelerated hematopoietic recovery in cyclophosphamide-treated mice. Assay of serum colony-stimulating activity (CSA) revealed that (a) cyclophosphamide treatment alone significantly increased the level of circulating CSA, (b) administration of a single dose of IL-1 to neutropenic mice induced an early, further increase in serum CSA, followed by depression, (c) a biphasic pattern of CSA response was also evident in mice repeatedly treated with IL-1. These results suggest that regulation of hematopoiesis may have an important role in the induction of antibacterial resistance in myelosuppressed hosts repeatedly treated with low dosages of IL-1. |
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Authors:
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F Campanile; L Binaglia; D Boraschi; A Tagliabue; M C Fioretti; P Puccetti |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Cellular immunology Volume: 128 ISSN: 0008-8749 ISO Abbreviation: Cell. Immunol. Publication Date: 1990 Jun |
Date Detail:
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Created Date: 1990-06-29 Completed Date: 1990-06-29 Revised Date: 2003-11-14 |
Medline Journal Info:
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Nlm Unique ID: 1246405 Medline TA: Cell Immunol Country: UNITED STATES |
Other Details:
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Languages: eng Pagination: 250-60 Citation Subset: IM |
Affiliation:
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Department of Experimental Medicine and Biochemical Sciences, University of Perugia, Italy. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Blood Bactericidal Activity* Colony-Forming Units Assay Cyclophosphamide / pharmacology Dose-Response Relationship, Drug Female Gentamicins / therapeutic use Hematopoiesis / drug effects Immunotherapy Interleukin-1 / pharmacology, therapeutic use* Leukocyte Count / drug effects Mice Mice, Inbred BALB C Neutrophils / immunology Pseudomonas Infections / therapy* Pseudomonas aeruginosa Recombinant Proteins Time Factors |
| Chemical | |
Reg. No./Substance:
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0/Gentamicins; 0/Interleukin-1; 0/Recombinant Proteins; 50-18-0/Cyclophosphamide |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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