| Antibacterial potential of sponge endosymbiont marine Enterobacter sp at Kavaratti Island, Lakshadweep archipelago. | |
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MedLine Citation:
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PMID: 22221759 Owner: NLM Status: In-Data-Review |
Abstract/OtherAbstract:
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OBJECTIVE: To isolate antibacterial potential of sponge endosymbiotic bacteria from marine sponges at Lakshadweep archipelago. Also to identify the potent bacteria by 16s rDNA sequencing and determine the antibacterial activity against clinical pathogens by MIC. METHODS: Sponge samples was collected from sub-tidal habitats at Kavaratti Island and identified. The endosymbiotic bacteria were isolated and selected potential bacteria which show antibacterial activity in preliminary screening against clinical pathogens Staphylococcus aureus (S. aureus), Escherichia coli (E. coli), Salmonella typhi (S. typhi), Klebsiella pneumoniea (K. pneumoniea) and Streptococcus sp. by disc diffusion assay. The crude extracts of potential bacteria LB3 was tested against clinical pathogens by MIC. The LB3 strain was identified by 16s rDNA sequencing, 1 111 bp was submitted in NCBI (HQ589912) and constructed phylogenetic tree. RESULTS: Sponge sample was identified as Dysidea granulosa (D. granulosa) and potential bacteria LB3 identified as Enterobacter sp TTAG. Preliminary screening of sponge isolates against clinical pathogens, LB3 strain was selected as potential producer of secondary metabolites and crude extract was implies on MIC of LB3 have confirmed with lowest concentration of 5.0 mg/mL in broth medium influence of crude extract on growth inhibitory activity after 5 h of incubation period and completed the inhibitory activity at 15 h. CONCLUSIONS: The present study concluded that phylogenetic analysis of endosymbiotic bacteria Enterobacter sp from sponge D. granulosa of Lakshadweep islands showed significant antibacterial activity against clinical bacterial pathogens. |
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Authors:
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Mohan Gopi; Subramanian Kumaran; Thipparamalai Thangappanpillai Ajith Kumar; Balaraman Deivasigamani; Kumarappan Alagappan; Srinivasan Guru Prasad |
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Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: Asian Pacific journal of tropical medicine Volume: 5 ISSN: 1995-7645 ISO Abbreviation: Asian Pac J Trop Med Publication Date: 2012 Feb |
Date Detail:
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Created Date: 2012-01-06 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 101533720 Medline TA: Asian Pac J Trop Med Country: Singapore |
Other Details:
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Languages: eng Pagination: 142-6 Citation Subset: IM |
Copyright Information:
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Copyright © 2012 Hainan Medical College. Published by Elsevier B.V. All rights reserved. |
Affiliation:
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CAS in Marine Biology, Faculty of Marine Sciences, Annamalai University, Parangipettai- 608 502, Tamil Nadu, India; CMLRE Field Research Station, Agatti Island, Lakshadweep- 682 553, India. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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