|Antibacterial and physical properties of calcium-phosphate and calcium-fluoride nanocomposites with chlorhexidine.|
|PMID: 22317794 Owner: NLM Status: MEDLINE|
|OBJECTIVES: Previous studies have developed calcium phosphate and fluoride releasing composites. Other studies have incorporated chlorhexidine (CHX) particles into dental composites. However, CHX has not been incorporated in calcium phosphate and fluoride composites. The objectives of this study were to develop nanocomposites containing amorphous calcium phosphate (ACP) or calcium fluoride (CaF(2)) nanoparticles and CHX particles, and investigate Streptococcus mutans biofilm formation and lactic acid production for the first time.
METHODS: Chlorhexidine was frozen via liquid nitrogen and ground to obtain a particle size of 0.62 μm. Four nanocomposites were fabricated with fillers of: nano ACP; nano ACP+10% CHX; nano CaF(2); nano CaF(2)+10% CHX. Three commercial materials were tested as controls: a resin-modified glass ionomer, and two composites. S. mutans live/dead assay, colony-forming unit (CFU) counts, biofilm metabolic activity, and lactic acid were measured.
RESULTS: Adding CHX fillers to ACP and CaF(2) nanocomposites greatly increased their antimicrobial capability. ACP and CaF(2) nanocomposites with CHX that were inoculated with S. mutans had a growth medium pH>6.5 after 3 d, while the control commercial composites had a cariogenic pH of 4.2. Nanocomposites with CHX reduced the biofilm metabolic activity by 10-20 folds and reduced the acid production, compared to the controls. CFU on nanocomposites with CHX were three orders of magnitude less than that on commercial composite. Mechanical properties of nanocomposites with CHX matched a commercial composite without fluoride.
SIGNIFICANCE: The novel calcium phosphate and fluoride nanocomposites could be rendered antibacterial with CHX to greatly reduce biofilm formation, acid production, CFU and metabolic activity. The antimicrobial and remineralizing nanocomposites with good mechanical properties may be promising for a wide range of tooth restorations with anti-caries capabilities.
|Lei Cheng; Michael D Weir; Hockin H K Xu; Alison M Kraigsley; Nancy J Lin; Sheng Lin-Gibson; Xuedong Zhou|
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|Type: Comparative Study; Journal Article; Research Support, N.I.H., Extramural; Research Support, Non-U.S. Gov't Date: 2012-02-06|
|Title: Dental materials : official publication of the Academy of Dental Materials Volume: 28 ISSN: 1879-0097 ISO Abbreviation: Dent Mater Publication Date: 2012 May|
|Created Date: 2012-04-09 Completed Date: 2012-09-25 Revised Date: 2014-09-14|
Medline Journal Info:
|Nlm Unique ID: 8508040 Medline TA: Dent Mater Country: England|
|Languages: eng Pagination: 573-83 Citation Subset: D|
|Copyright © 2012 Academy of Dental Materials. All rights reserved.|
|APA/MLA Format Download EndNote Download BibTex|
Bacterial Load / drug effects
Biofilms / drug effects
Bisphenol A-Glycidyl Methacrylate / chemistry
Calcium Fluoride / chemistry, pharmacology*
Calcium Phosphates / chemistry, pharmacology*
Chlorhexidine / chemistry, pharmacology*
Composite Resins / chemistry, pharmacology*
Dental Materials / chemistry, pharmacology*
Glass / chemistry
Glass Ionomer Cements / chemistry
Lactic Acid / analysis
Microbial Viability / drug effects
Microscopy, Electron, Scanning
Nanocomposites / chemistry*
Polyethylene Glycols / chemistry
Polymethacrylic Acids / chemistry
Resin Cements / chemistry
Streptococcus mutans / drug effects
|R01 DE017974/DE/NIDCR NIH HHS; R01 DE017974-03/DE/NIDCR NIH HHS; R01 DE017974-04/DE/NIDCR NIH HHS; R01 DE14190/DE/NIDCR NIH HHS; R01 DE17974/DE/NIDCR NIH HHS; Y1-DE-7005-01/DE/NIDCR NIH HHS|
|0/Anti-Bacterial Agents; 0/Calcium Phosphates; 0/Composite Resins; 0/Dental Materials; 0/Glass Ionomer Cements; 0/Polyethylene Glycols; 0/Polymethacrylic Acids; 0/Resin Cements; 0/amorphous calcium phosphate; 109-16-0/triethylene glycol dimethacrylate; 33X04XA5AT/Lactic Acid; 454I75YXY0/Bisphenol A-Glycidyl Methacrylate; O3B55K4YKI/Calcium Fluoride; R4KO0DY52L/Chlorhexidine|
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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