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Antibacterial and antibiofilm effects of iron chelators against Prevotella intermedia.
MedLine Citation:
PMID:  23329319     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
Prevotella intermedia, a major periodontopathogen, has been shown to be resistant to many antibiotics. In the present study, we examined the effect of FDA-approved iron chelators deferoxamine (DFO) and deferasirox (DFRA) against planktonic and biofilm cells of P. intermedia in order to evaluate the possibility of the iron chelators as alternative controlling agents against P. intermedia. DFRA showed strong antimicrobial activity (MIC and MBC values of 0.16 mg/ml) against planktonic P. intermedia. At sub-MICs, DFRA partially inhibited the bacterial growth and considerably prolonged the bacterial doubling time. DFO was unable to completely inhibit the bacterial growth in the concentration range tested and was not bactericidal. Crystal violet binding assay for the assessment of biofilm formation by P. intermedia showed that DFRA significantly decreased the biofilm-forming activity as well as the biofilm formation, while DFO was less effective. DFRA was chosen for further study. In the adenosine triphosphate (ATP)-bioluminescent assay, which reflects viable cell counts, sub-MICs of DFRA significantly decreased the bioactivity of biofilms in a concentration-dependent manner. Under the scanning electron microscope, P. intermedia cells in DFRA-treated biofilm were significantly elongated compared to the bacterial cells in untreated biofilm. Further experiments are necessary to show that iron chelators may be used as a therapeutic agent for periodontal disease.
Authors:
Ji-Hoi Moon; Cheul Kim; Hee-Su Lee; Sung-Woon Kim; Jin-Yong Lee
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2013-1-17
Journal Detail:
Title:  Journal of medical microbiology     Volume:  -     ISSN:  1473-5644     ISO Abbreviation:  J. Med. Microbiol.     Publication Date:  2013 Jan 
Date Detail:
Created Date:  2013-1-18     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  0224131     Medline TA:  J Med Microbiol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
1 School of Dentistry, Kyung Hee University;
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