| Antibacterial activity of sphingoid bases and fatty acids against Gram-positive and Gram-negative bacteria. | |
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MedLine Citation:
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PMID: 22155833 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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There is growing evidence that the role of lipids in innate immunity is more important than previously realized. How lipids interact with bacteria to achieve a level of protection, however, is still poorly understood. To begin to address the mechanisms of antibacterial activity, we determined MICs and minimum bactericidal concentrations (MBCs) of lipids common to the skin and oral cavity--the sphingoid bases D-sphingosine, phytosphingosine, and dihydrosphingosine and the fatty acids sapienic acid and lauric acid--against four Gram-negative bacteria and seven Gram-positive bacteria. Exact Kruskal-Wallis tests of these values showed differences among lipid treatments (P < 0.0001) for each bacterial species except Serratia marcescens and Pseudomonas aeruginosa. D-sphingosine (MBC range, 0.3 to 19.6 μg/ml), dihydrosphingosine (MBC range, 0.6 to 39.1 μg/ml), and phytosphingosine (MBC range, 3.3 to 62.5 μg/ml) were active against all bacteria except S. marcescens and P. aeruginosa (MBC > 500 μg/ml). Sapienic acid (MBC range, 31.3 to 375.0 μg/ml) was active against Streptococcus sanguinis, Streptococcus mitis, and Fusobacterium nucleatum but not active against Escherichia coli, Staphylococcus aureus, S. marcescens, P. aeruginosa, Corynebacterium bovis, Corynebacterium striatum, and Corynebacterium jeikeium (MBC > 500 μg/ml). Lauric acid (MBC range, 6.8 to 375.0 μg/ml) was active against all bacteria except E. coli, S. marcescens, and P. aeruginosa (MBC > 500 μg/ml). Complete killing was achieved as early as 0.5 h for some lipids but took as long as 24 h for others. Hence, sphingoid bases and fatty acids have different antibacterial activities and may have potential for prophylactic or therapeutic intervention in infection. |
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Authors:
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Carol L Fischer; David R Drake; Deborah V Dawson; Derek R Blanchette; Kim A Brogden; Philip W Wertz |
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Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural Date: 2011-12-12 |
Journal Detail:
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Title: Antimicrobial agents and chemotherapy Volume: 56 ISSN: 1098-6596 ISO Abbreviation: Antimicrob. Agents Chemother. Publication Date: 2012 Mar |
Date Detail:
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Created Date: 2012-02-16 Completed Date: 2012-08-24 Revised Date: 2013-05-22 |
Medline Journal Info:
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Nlm Unique ID: 0315061 Medline TA: Antimicrob Agents Chemother Country: United States |
Other Details:
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Languages: eng Pagination: 1157-61 Citation Subset: IM |
Affiliation:
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Dows Institute for Dental Research, College of Dentistry, University of Iowa, Iowa City, Iowa, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Anti-Bacterial Agents
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immunology,
pharmacology* Gram-Negative Bacteria / drug effects*, growth & development Gram-Negative Bacterial Infections / immunology, prevention & control Gram-Positive Bacteria / drug effects*, growth & development Gram-Positive Bacterial Infections / immunology, prevention & control Humans Immunity, Innate* Lauric Acids / immunology, metabolism, pharmacology Microbial Sensitivity Tests Mouth / immunology*, microbiology Palmitic Acids / immunology, metabolism, pharmacology Skin / immunology*, microbiology Species Specificity Sphingosine / analogs & derivatives, immunology, pharmacology, secretion |
| Grant Support | |
ID/Acronym/Agency:
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R01 DE014390/DE/NIDCR NIH HHS; R01 DE014390-09/DE/NIDCR NIH HHS; R01 DE018032/DE/NIDCR NIH HHS; R01DEO14390//PHS HHS |
| Chemical | |
Reg. No./Substance:
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0/Anti-Bacterial Agents; 0/Lauric Acids; 0/Palmitic Acids; 1160N9NU9U/lauric acid; 123-78-4/Sphingosine; 554-62-1/phytosphingosine; 6460S1EJ28/hexadecenoic acid; 764-22-7/safingol |
| Comments/Corrections | |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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