| Antibacterial efficacy of silver-impregnated polyelectrolyte multilayers immobilized on a biological dressing in a murine wound infection model. | |
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MedLine Citation:
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PMID: 22609841 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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OBJECTIVE: To investigate the antibacterial effect of augmenting a biological dressing with polymer films containing silver nanoparticles. BACKGROUND: Biological dressings, such as Biobrane, are commonly used for treating partial-thickness wounds and burn injuries. Biological dressings have several advantages over traditional wound dressings. However, as many as 19% of wounds treated with Biobrane become infected, and, once infected, the Biobrane must be removed and a traditional dressing approach should be employed. Silver is a commonly used antimicrobial in wound care products, but current technology uses cytotoxic concentrations of silver in these dressings. We have developed a novel and facile technology that allows immobilization of bioactive molecules on the surfaces of soft materials, demonstrated here by augmentation of Biobrane with nanoparticulate silver. Surfaces modified with nanometer-thick polyelectrolyte multilayers (PEMs) impregnated with silver nanoparticles have been shown previously to result in in vitro antibacterial activity against Staphylococcus epidermidis at loadings of silver that are noncytotoxic. METHODS: We demonstrated that silver-impregnated PEMs can be nondestructively immobilized onto the surface of Biobrane (Biobrane-Ag) and determined the in vitro antibacterial activity of Biobrane-Ag with Staphylococcus aureus. In this study, we used an in vivo wound infection model in mice induced by topical inoculation of S aureus onto full-thickness 6-mm diameter wounds. After 72 hours, bacterial quantification was performed. RESULTS: Wounds treated with Biobrane-Ag had significantly (P < 0.001) fewer colony-forming units than wounds treated with unmodified Biobrane (more than 4 log10 difference). CONCLUSIONS: The results of our study indicate that immobilizing silver-impregnated PEMs on the wound-contact surface of Biobrane significantly reduces bacterial bioburden in full-thickness murine skin wounds. Further research will investigate whether this construct can be considered for human use. |
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Authors:
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Kathleen M Guthrie; Ankit Agarwal; Dana S Tackes; Kevin W Johnson; Nicholas L Abbott; Christopher J Murphy; Charles J Czuprynski; Patricia R Kierski; Michael J Schurr; Jonathan F McAnulty |
Publication Detail:
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Type: Journal Article; Research Support, N.I.H., Extramural |
Journal Detail:
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Title: Annals of surgery Volume: 256 ISSN: 1528-1140 ISO Abbreviation: Ann. Surg. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-07-13 Completed Date: 2012-09-25 Revised Date: 2013-04-16 |
Medline Journal Info:
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Nlm Unique ID: 0372354 Medline TA: Ann Surg Country: United States |
Other Details:
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Languages: eng Pagination: 371-7 Citation Subset: AIM; IM |
Affiliation:
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School of Veterinary Medicine, Department of Surgical Sciences, University of Wisconsin, Madison, WI 53706, USA. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Biological Dressings* Coated Materials, Biocompatible / chemistry, therapeutic use* Disease Models, Animal Metal Nanoparticles Mice Occlusive Dressings* Polymers / chemistry Silver / chemistry Tissue Engineering / methods* Wound Healing |
| Grant Support | |
ID/Acronym/Agency:
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R01 CA108467/CA/NCI NIH HHS; R21 AI092004/AI/NIAID NIH HHS; RC2 AR058971/AR/NIAMS NIH HHS |
| Chemical | |
Reg. No./Substance:
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0/Biobrane; 0/Coated Materials, Biocompatible; 0/Polymers; 7440-22-4/Silver |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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