Document Detail


Antibacterial, Anti-Chlamydial, and Cytotoxic Activities of a Marine Snail (Hexaplex trunculus) Phospholipase A2: an In Vitro Study.
MedLine Citation:
PMID:  22899013     Owner:  NLM     Status:  Publisher    
Abstract/OtherAbstract:
In order to report pharmacological characterization of marine snail (Hexaplex trunculus) hepatopancreatic phospholipase A(2) (mSDPLA(2)), we have talked for the first time the antimicrobial activity against different pathogenic bacterial strains, anti-chlamydial activity as well as its cytotoxic activity against McCoy cell lines. mSDPLA(2), showed a high level of activity towards Gram-positive bacteria as Staphylococcus aureus and Staphylococcus epidermidis. Whereas Gram-negative bacteria, unfortunately, exhibited a higher resistance, mSDPLA(2) was also found to have a strong cytotoxic activity, causing significant morphological alterations of the McCoy cell lines surfaces and to be a hinder to the proliferation. Moreover, mSDPLA(2) proved to have a very potent anti-chlamydial activity. Over 95 % inhibition of chlamydial inclusions were obtained at a concentration of 10 μg/ml of mSDPLA(2) after 24 h postinfection. Interestingly, at a concentration of 10 μg/ml of mSDPLA(2), the proliferation of McCoy cells was not affected. Approximately 50 % inhibition of cell growth was obtained with a concentration of 37 μg/mL of mSDPLA(2). mSDPLA(2) could be considered as an excellent candidate for the development of a new anti-infective agent. This enzyme showed significant antimicrobial activities.
Authors:
Zied Zarai; Houda Gharsallah; Adnane Hammami; Hafedh Mejdoub; Sofiane Bezzine; Youssef-Talel Gargouri
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Publication Detail:
Type:  JOURNAL ARTICLE     Date:  2012-8-17
Journal Detail:
Title:  Applied biochemistry and biotechnology     Volume:  -     ISSN:  1559-0291     ISO Abbreviation:  Appl. Biochem. Biotechnol.     Publication Date:  2012 Aug 
Date Detail:
Created Date:  2012-8-17     Completed Date:  -     Revised Date:  -    
Medline Journal Info:
Nlm Unique ID:  8208561     Medline TA:  Appl Biochem Biotechnol     Country:  -    
Other Details:
Languages:  ENG     Pagination:  -     Citation Subset:  -    
Affiliation:
Laboratoire de biochimie et de génie enzymatique des lipases, Université de Sfax-Tunisie, ENIS route de Soukra, BPW, 1173, Sfax, Tunisia.
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