| Antibacterial, Anti-Chlamydial, and Cytotoxic Activities of a Marine Snail (Hexaplex trunculus) Phospholipase A2: an In Vitro Study. | |
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MedLine Citation:
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PMID: 22899013 Owner: NLM Status: Publisher |
Abstract/OtherAbstract:
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In order to report pharmacological characterization of marine snail (Hexaplex trunculus) hepatopancreatic phospholipase A(2) (mSDPLA(2)), we have talked for the first time the antimicrobial activity against different pathogenic bacterial strains, anti-chlamydial activity as well as its cytotoxic activity against McCoy cell lines. mSDPLA(2), showed a high level of activity towards Gram-positive bacteria as Staphylococcus aureus and Staphylococcus epidermidis. Whereas Gram-negative bacteria, unfortunately, exhibited a higher resistance, mSDPLA(2) was also found to have a strong cytotoxic activity, causing significant morphological alterations of the McCoy cell lines surfaces and to be a hinder to the proliferation. Moreover, mSDPLA(2) proved to have a very potent anti-chlamydial activity. Over 95 % inhibition of chlamydial inclusions were obtained at a concentration of 10 μg/ml of mSDPLA(2) after 24 h postinfection. Interestingly, at a concentration of 10 μg/ml of mSDPLA(2), the proliferation of McCoy cells was not affected. Approximately 50 % inhibition of cell growth was obtained with a concentration of 37 μg/mL of mSDPLA(2). mSDPLA(2) could be considered as an excellent candidate for the development of a new anti-infective agent. This enzyme showed significant antimicrobial activities. |
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Authors:
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Zied Zarai; Houda Gharsallah; Adnane Hammami; Hafedh Mejdoub; Sofiane Bezzine; Youssef-Talel Gargouri |
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Publication Detail:
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Type: JOURNAL ARTICLE Date: 2012-8-17 |
Journal Detail:
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Title: Applied biochemistry and biotechnology Volume: - ISSN: 1559-0291 ISO Abbreviation: Appl. Biochem. Biotechnol. Publication Date: 2012 Aug |
Date Detail:
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Created Date: 2012-8-17 Completed Date: - Revised Date: - |
Medline Journal Info:
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Nlm Unique ID: 8208561 Medline TA: Appl Biochem Biotechnol Country: - |
Other Details:
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Languages: ENG Pagination: - Citation Subset: - |
Affiliation:
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Laboratoire de biochimie et de génie enzymatique des lipases, Université de Sfax-Tunisie, ENIS route de Soukra, BPW, 1173, Sfax, Tunisia. |
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From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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