| Anti-tumor effects of 25-hydroxycholesterol and low-dose recombinant tumor necrosis factor-alpha on rat liver tumorigenesis: modulated differentiation therapy for hepatocellular carcinoma. | |
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MedLine Citation:
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PMID: 10762641 Owner: NLM Status: MEDLINE |
Abstract/OtherAbstract:
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Previously, we have demonstrated that treatment of rat AH136B ascites hepatoma cells with 25-hydroxycholesterol (25-OH) induces apoptosis. In this study, to elucidate whether modulated differentiation therapy may be useful in treating patients with hepatoma, we assayed the effects of 25-OH and/or tumor necrosis factor-alpha (TNF) on rat AH136B ascites hepatoma cells in vivo. Here we show that a high concentration of TNF (250 U/ml) mediated a stronger cytocidal effect than a low concentration of TNF (25 U/ml) in vitro. Flow cytometric DNA analysis also showed that treatment of cells with the high concentration of TNF (250 U/ml) increased the percentage of AH136B cells in the G0/G1 phase, while 25 U/ml of TNF did not cause any marked change in cell kinetics. In in vivo experiments, 25-OH (80 microg/rat) and TNF (500 U/rat) were administered after transplanting AH136B cells into Donryu rats intraperitoneally. We found that tumor development was completely inhibited by this treatment in 3 of 9 rats, and their 40-day survival rate was 44%; in contrast, rats administered 25-OH (80 microg/rat) alone or TNF (500 U/ml) alone developed peritonitis carcinomatosa, and died within 13 days of inoculation. These findings suggest that local combined treatment with 25-hydroxycholesterol and low-dose TNF can induce synergistic anti-tumor effects. |
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Authors:
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S Yokoyama; F Nozawa; S Tanaka; S Muneyuki; M Ogawa |
Publication Detail:
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Type: Journal Article |
Journal Detail:
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Title: International journal of oncology Volume: 16 ISSN: 1019-6439 ISO Abbreviation: Int. J. Oncol. Publication Date: 2000 May |
Date Detail:
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Created Date: 2000-05-22 Completed Date: 2000-05-22 Revised Date: 2006-04-24 |
Medline Journal Info:
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Nlm Unique ID: 9306042 Medline TA: Int J Oncol Country: GREECE |
Other Details:
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Languages: eng Pagination: 1029-33 Citation Subset: IM |
Affiliation:
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Department of Surgery II, Kumamoto University School of Medicine, Kumamoto 860-0811, Japan. |
Export Citation:
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APA/MLA Format Download EndNote Download BibTex |
| MeSH Terms | |
Descriptor/Qualifier:
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Animals Antineoplastic Combined Chemotherapy Protocols / therapeutic use* Carcinoma, Hepatocellular / prevention & control* Hydroxycholesterols / administration & dosage* Liver Neoplasms / prevention & control* Male Neoplasm Transplantation Rats Recombinant Proteins / administration & dosage* Tumor Cells, Cultured Tumor Necrosis Factor-alpha / administration & dosage* |
| Chemical | |
Reg. No./Substance:
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0/Hydroxycholesterols; 0/Recombinant Proteins; 0/Tumor Necrosis Factor-alpha; 2140-46-7/25-hydroxycholesterol |
From MEDLINE®/PubMed®, a database of the U.S. National Library of Medicine
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