Document Detail

Anti-tumor activity of dehydroxymethylepoxyquinomicin against human oral squamous cell carcinoma cell lines in vitro and in vivo.
MedLine Citation:
PMID:  21459660     Owner:  NLM     Status:  MEDLINE    
Several reports have indicated that nuclear factor-kappa B (NF-κB) is constitutively activated in a variety of cancer cells including human oral squamous carcinoma cells, and play a key role in their growth and survival. Recent studies report that NF-κB inhibitor, dehydroxymethylepoxyquinomicin (DHMEQ), inhibits proliferation and induces apoptosis in prostate cancer cell lines. However this anti-tumor effects are still unknown in end human oral squamous carcinoma cells. In the present study, we investigated the effects of DHMEQ on oral squamous carcinoma cell (OSCC) lines in vitro and in vivo. Human OSCC cell lines (HSC-3, SAS) were treated with DHMEQ and examined for cell viability by MTT assay, cell cycle distribution by flow-cytometry, apoptosis by TUNEL assay, and protein expression by western blotting, respectively. In vivo activities were also investigated in a mouse xenograft model. DHMEQ inhibited growth of two OSCC cell lines in a dose-dependent manner measured by MTT assay. A flow cytometric analysis demonstrated that treatment with DHMEQ induced accumulation in sub-G1 phase. TUNEL assay showed that DHMEQ induced DNA fragmentation. Protein expression by western blotting analysis revealed that DHMEQ induced nuclear down regulation of Survivin, cIAP-1, and cIAP-2. In nude mice, DHMEQ inhibited growth of OSCC without major toxic side effects. The present results demonstrated that administration of DHMEQ is suggested to be a novel anti-tumor approach to the treatment of OSCC.
Arisa Yasuda; Seiji Kondo; Tatsuhito Nagumo; Hikari Tsukamoto; Yoshiki Mukudai; Kazuo Umezawa; Satoru Shintani
Publication Detail:
Type:  Journal Article; Research Support, Non-U.S. Gov't     Date:  2011-04-02
Journal Detail:
Title:  Oral oncology     Volume:  47     ISSN:  1879-0593     ISO Abbreviation:  Oral Oncol.     Publication Date:  2011 May 
Date Detail:
Created Date:  2011-05-20     Completed Date:  2011-08-24     Revised Date:  2014-07-31    
Medline Journal Info:
Nlm Unique ID:  9709118     Medline TA:  Oral Oncol     Country:  England    
Other Details:
Languages:  eng     Pagination:  334-9     Citation Subset:  IM    
Copyright Information:
Copyright © 2011 Elsevier Ltd. All rights reserved.
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MeSH Terms
Antineoplastic Agents / pharmacology*
Benzamides / pharmacology*
Carcinoma, Squamous Cell / drug therapy*,  mortality
Cell Cycle / drug effects*,  genetics
Cell Line, Tumor
Cyclohexanones / pharmacology*
Dose-Response Relationship, Drug
Flow Cytometry
Inhibitor of Apoptosis Proteins / metabolism
Mouth Neoplasms / drug therapy*,  pathology
NF-kappa B / antagonists & inhibitors*,  metabolism
Reverse Transcriptase Polymerase Chain Reaction
Tumor Cells, Cultured
Reg. No./Substance:
0/Antineoplastic Agents; 0/Benzamides; 0/Cyclohexanones; 0/Inhibitor of Apoptosis Proteins; 0/NF-kappa B; 0/dehydroxymethylepoxyquinomicin

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